Biomaterial Database

Immunohistochemical study of hard tissue formation in the rat pulp cavity after tooth replantation. 2007

Chen Zhao, and Akihiro Hosoya, and Hiroshi Kurita, and Tao Hu, and Toru Hiraga, and Tadashi Ninomiya, and Kunihiko Yoshiba, and Nagako Yoshiba, and Masafumi Takahashi, and Kenji Kurashina, and Hidehiro Ozawa, and Hiroaki Nakamura

Department of Dental and Oral Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Japan.

While mineralized tissue is formed in the pulp cavity after tooth replantation or transplantation, little is known of this hard tissue formation. Therefore, we conducted histological and immunohistochemical evaluations of hard tissue formed in the pulp of rat maxillary molars after tooth replantation. At 5 days after replantation, degenerated odontoblasts were lining the pulp cavity. At 14 days, dentin- or bone-like tissue was present in the pulp cavity. Immunoreactivity for osteopontin (OPN) and bone sialoprotein (BSP) was strong in the bone-like tissue, but weak in the dentin-like tissue. Conversely, dentin sialoprotein (DSP) was localized in the dentin-like tissue, but not in the bone-like tissue. Cells positive for BMP4, Smad4, Runx2, and Osterix were found around the blood vessels of the root apex at 5 days. At 14 days, these cells were also localized around the bone-like tissue. Cells expressing alpha-smooth muscle actin (SMA) were seen around the newly formed bone-like tissue, whereas no such cells were found around the newly formed dentin-like tissue. In an experiment involving the transplantation of a green fluorescent protein (GFP)-transgenic rat tooth into a wild-type rat tooth socket, GFP-positive cells were detected on the surface of the bone-like tissue and over all dentin-like tissue. These results indicate that the original pulp cells had the ability to differentiate into osteoblast-like cells as well as into odontoblast-like cells.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009804	Odontoblasts	The mesenchymal cells which line the DENTAL PULP CAVITY and produce DENTIN. They have a columnar morphology in the coronal pulp but are cuboidal in the root pulp, or when adjacent to tertiary dentin.	Odontoblast
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D003739	Dental Cementum	The bonelike rigid connective tissue covering the root of a tooth from the cementoenamel junction to the apex and lining the apex of the root canal, also assisting in tooth support by serving as attachment structures for the periodontal ligament. (Jablonski, Dictionary of Dentistry, 1992)	Cementoblasts,Cementum,Cementoblast,Cementum, Dental
D003784	Dental Pulp Calcification	CALCINOSIS of the DENTAL PULP or ROOT CANAL.	Dental Pulp Stones,Denticle,Anomalous Dysplasia of Dentin,Calcification, Dental Pulp,Coronal Dentin Dysplasia,Dental Pulp Stone,Denticles,Dentin Dyspalsia, Shields Type 2,Dentin Dysplasia, Coronal,Dentin Dysplasia, Shields Type II,Dentin Dysplasia, Type II,Pulp Calcification, Dental,Pulp Stone, Dental,Pulp Stones,Pulp Stones, Dental,Pulpal Dysplasia,Stone, Dental Pulp,Stones, Dental Pulp,Calcifications, Dental Pulp,Coronal Dentin Dysplasias,Dental Pulp Calcifications,Dentin Anomalous Dysplasia,Dentin Anomalous Dysplasias,Dentin Dysplasias, Coronal,Dysplasia, Coronal Dentin,Dysplasia, Pulpal,Dysplasias, Coronal Dentin,Dysplasias, Pulpal,Pulp Calcifications, Dental,Pulp Stone,Pulpal Dysplasias,Stone, Pulp,Stones, Pulp
D003804	Dentin	The hard portion of the tooth surrounding the pulp, covered by enamel on the crown and cementum on the root, which is harder and denser than bone but softer than enamel, and is thus readily abraded when left unprotected. (From Jablonski, Dictionary of Dentistry, 1992)	Dentine,Dentines,Dentins
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014090	Tooth Replantation	Reinsertion of a tooth into the alveolus from which it was removed or otherwise lost.	Reimplantation, Tooth,Replantation, Tooth,Tooth Reimplantation,Reimplantations, Tooth,Replantations, Tooth,Tooth Reimplantations,Tooth Replantations
D014092	Tooth Root	The part of a tooth from the neck to the apex, embedded in the alveolar process and covered with cementum. A root may be single or divided into several branches, usually identified by their relative position, e.g., lingual root or buccal root. Single-rooted teeth include mandibular first and second premolars and the maxillary second premolar teeth. The maxillary first premolar has two roots in most cases. Maxillary molars have three roots. (Jablonski, Dictionary of Dentistry, 1992, p690)	Root, Tooth,Roots, Tooth,Tooth Roots
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus