BIOMAT Database Logo BIOMAT Database Logo

Gallus expression in situ hybridization analysis: a chicken embryo gene expression database. 2007

P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Department of Cell Biology and Anatomy, University of Arizona, Tucson 85724, USA. pba@email.arizona.edu

With sequencing of the chicken genome largely completed, significant effort is focusing on gene annotation, including acquiring information about the patterns of gene expression. The chicken embryo is ideally suited to provide detailed temporal and spatial expression information through in situ hybridization gene expression analysis in vivo. We have developed the Gallus expression in situ hybridization analysis (GEISHA) database and user interface (http://geisha.arizona.edu) to serve as a centralized repository of in situ hybridization photos and metadata from chicken embryos. This report describes the design and implementation the GEISHA database and Web site and illustrates its usefulness for researchers in the biomedical and poultry science communities. Results from a recent comprehensive expression analysis of microRNA expression in chicken embryos are also presented.

UI MeSH Term Description Entries
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017403 In Situ Hybridization A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. Hybridization in Situ,Hybridization, In Situ,Hybridizations, In Situ,In Situ Hybridizations
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays
D030541 Databases, Genetic Databases devoted to knowledge about specific genes and gene products. Genetic Databases,Genetic Sequence Databases,OMIM,Online Mendelian Inheritance In Man,Genetic Data Banks,Genetic Data Bases,Genetic Databanks,Genetic Information Databases,Bank, Genetic Data,Banks, Genetic Data,Data Bank, Genetic,Data Banks, Genetic,Data Base, Genetic,Data Bases, Genetic,Databank, Genetic,Databanks, Genetic,Database, Genetic,Database, Genetic Information,Database, Genetic Sequence,Databases, Genetic Information,Databases, Genetic Sequence,Genetic Data Bank,Genetic Data Base,Genetic Databank,Genetic Database,Genetic Information Database,Genetic Sequence Database,Information Database, Genetic,Information Databases, Genetic,Sequence Database, Genetic,Sequence Databases, Genetic
D035683 MicroRNAs Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing. RNA, Small Temporal,Small Temporal RNA,miRNA,stRNA,Micro RNA,MicroRNA,Primary MicroRNA,Primary miRNA,miRNAs,pre-miRNA,pri-miRNA,MicroRNA, Primary,RNA, Micro,Temporal RNA, Small,miRNA, Primary,pre miRNA,pri miRNA

Related Publications

P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Temporospatial expression of Dmrt1 in chicken urogenital system (Gallus gallus) using whole mount in situ hybridization.
June 2013, Acta biologica Hungarica,
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Gonadotropins regulate cadherin expression in the ovary of the chicken embryo (Gallus gallus).
December 2011, General and comparative endocrinology,
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Ontogeny of renin gene expression in the chicken, Gallus gallus.
September 2020, General and comparative endocrinology,
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
GEISHA, a whole-mount in situ hybridization gene expression screen in chicken embryos.
March 2004, Developmental dynamics : an official publication of the American Association of Anatomists,
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
DNA methylation analysis of the gene CDKN2B in Gallus gallus (chicken).
January 2013, Folia biologica,
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Gene expression analysis in cartilage by in situ hybridization.
January 2004, Methods in molecular medicine,
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Gene expression analysis in developing embryos: in situ hybridization.
January 2012, Methods in molecular biology (Clifton, N.J.),
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Gene expression analysis by in situ hybridization. Radioactive probes.
January 2000, Methods in molecular biology (Clifton, N.J.),
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
Maternal diet influences gene expression in intestine of offspring in chicken (Gallus gallus).
December 2006, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology,
P B Antin, and S Kaur, and S Stanislaw, and S Davey, and J H Konieczka, and T A Yatskievych, and D K Darnell
In vivo estrogen synthesis by the developing chicken (Gallus gallus) embryo.
February 1983, General and comparative endocrinology,
Copied contents to your clipboard!