Biomaterial Database

Developmental potentiality of embryos cultured under low oxygen tension with superoxide dismutase. 1991

Y Umaoka, and Y Noda, and K Narimoto, and T Mori

Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.

A low-oxygen culture of preimplantation embryos with superoxide dismutase (SOD) has been demonstrated to produce a marked increase in the blastocyst formation. In this study, we examined the effects of low oxygen-SOD cultures of embryos on the potentiality of embryonic development after intrauterine transplantation and the reproductive ability of the offspring. Mouse pronuclear embryos were cultured in Biggers-Whitten-Whittingham's solution containing 0.3% bovine serum albumin and 500 micrograms/ml SOD under a low-oxygen condition (5% O2, 5% CO2, 90% N2), and 37.2% (110/296) of the embryos developed to blastocysts, with a significant difference from the 1.3% (3/231) under an atmospheric oxygen condition without SOD (P less than 0.01). The blastocysts obtained under a low oxygen-SOD culture condition were transplanted by direct insertion into the uterus of pseudopregnant females 3 days after infertile coitus. The implantation rate was 65.0% (65/100), and the embryo viability rate was 46.0% (46/100). In the control group, in which in vivo developed blastocysts were transplanted into 3-day pseudopregnant females, the implantation rate was 72.5% (81/112), and the embryo viability rate was 51.8% (58/112). The differences between the two groups were not significant. Significant differences also were not observed in either the body weight of pups at birth and 35 days after birth or the mean number of pups delivered by successive mating of the offspring between the two groups.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D010100	Oxygen	An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.	Dioxygen,Oxygen-16,Oxygen 16
D010313	Partial Pressure	The pressure that would be exerted by one component of a mixture of gases if it were present alone in a container. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Partial Pressures,Pressure, Partial,Pressures, Partial
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D001757	Blastomeres	Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells.	Blastocytes,Blastocyte,Blastomere
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004622	Embryo, Mammalian	The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.	Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D004624	Embryo Transfer	The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.	Blastocyst Transfer,Tubal Embryo Transfer,Tubal Embryo Stage Transfer,Embryo Transfers,Transfer, Embryo,Transfers, Embryo
D005260	Female		Females
D005314	Embryonic and Fetal Development	Morphological and physiological development of EMBRYOS or FETUSES.	Embryo and Fetal Development,Prenatal Programming,Programming, Prenatal
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia