[Alphavirus replicon-vectored plasmid DNA-based vaccine elicits protective immunity against classical swine fever virus]. 2007

Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
College of Veterinary Sciences, Northeast Agricultural University, Harbin 150030, China.

We have shown previously that a Semliki Forest virus (SFV) replicon vectored DNA vaccine (pSFV1CS-E2) expressing the E2 glycoprotein of classical swine fever virus (CSFV) conferred full protection for pigs immunized three times with 600 microg of the vaccine. This study aims to evaluate the efficacy of the DNA vaccine with lower dosage and fewer inoculations. Pigs were immunized twice with 100 microg pSFV1CS-E2 (n = 5) or control plasmid pSFV1CS (n = 3), respectively. Pigs immunized with pSFV1CS-E2 developed high titers of specific neutralizing antibodies against CSFV after the booster, and the antibody titers increased rapidly upon challenge. The immunized animals showed no clinical symptoms except short-term fever and low-level viremia, whereas the control pigs immunized with the control plasmid produced no detectable antibody before challenge and showed obvious clinical signs following challenge, and 2 pigs died on 10 or 11 days post-challenge. All control animals developed extended viremia as detected by nested RT-PCR and real-time RT-PCR. Severe pathologic lesions typical of CSFV infection were observed at necropsy. We conclude that the alphavirus replicon-vectored DNA-based vaccine can be potential marker vaccine against CSFV.

UI MeSH Term Description Entries
D007114 Immunization Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow). Immunologic Stimulation,Immunostimulation,Sensitization, Immunologic,Variolation,Immunologic Sensitization,Immunological Stimulation,Sensitization, Immunological,Stimulation, Immunologic,Immunizations,Immunological Sensitization,Immunological Sensitizations,Immunological Stimulations,Sensitizations, Immunological,Stimulation, Immunological,Stimulations, Immunological,Variolations
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D012093 Replicon Any DNA sequence capable of independent replication or a molecule that possesses a REPLICATION ORIGIN and which is therefore potentially capable of being replicated in a suitable cell. (Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Replication Unit,Replication Units,Replicons,Unit, Replication,Units, Replication
D001831 Body Temperature The measure of the level of heat of a human or animal. Organ Temperature,Body Temperatures,Organ Temperatures,Temperature, Body,Temperature, Organ,Temperatures, Body,Temperatures, Organ
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D006691 Classical Swine Fever An acute, highly contagious disease affecting swine of all ages and caused by the CLASSICAL SWINE FEVER VIRUS. It has a sudden onset with high morbidity and mortality. Hog Cholera,Swine Fever,Swine Fever, Classical,Cholera, Hog
D006692 Classical Swine Fever Virus A species of the PESTIVIRUS genus causing exceedingly contagious and fatal hemorrhagic disease of swine. Hog Cholera Virus,Swine Fever Virus,Swine Fever Virus, Classical,Cholera Virus, Hog
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D012672 Semliki forest virus A species of ALPHAVIRUS isolated in central, eastern, and southern Africa.

Related Publications

Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
October 2011, Vaccine,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
September 2010, Veterinary immunology and immunopathology,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
May 2012, Vaccine,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
January 2023, Frontiers in immunology,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
November 2009, Vaccine,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
September 2009, Epidemiology and infection,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
October 1998, Virology,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
July 2014, PLoS neglected tropical diseases,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
January 2007, Vaccine,
Na Li, and Jian-Jun Zhao, and He-Ping Zhao, and Yuan Sun, and Qing-Hu Zhu, and Guang-Zhi Tong, and Hua-Ji Qiu
January 2001, Acta veterinaria Hungarica,
Copied contents to your clipboard!