Simultaneous determination of nitroimidazole residues in honey samples by high-performance liquid chromatography with ultraviolet detection. 2007

Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Apiculture Research Institute, Beeproduct Quality Supervision and Testing Center, Beijing 100093, People's Republic of China.

A rapid and sensitive high-performance liquid chromatography (LC) method was developed for the simultaneous determination of metronidazole (MNZ), dimetridazole (DMZ), ronidazole (RNZ), tinidazole (TNZ), and 2-hydroxymethyl-1-methyl-5-nitroimidazole (HMMNI) in honey. After extraction with ethyl acetate and evaporation, the residue containing the nitroimidazoles was dissolved in ethyl acetate-hexane and subjected to solid-phase extraction cleanup by amine extraction columns. The effluent was evaporated to dryness, and residues were dissolved and determined by LC with an ultraviolet detector set at 315 nm. The limits of detection were 1.0-2.0 ng/g for MNZ, DMZ, RNZ, TNZ, and HMMNI in honey. Average recoveries ranged from 71.5-101.4% in honey fortified at 10, 20, 50, and 100 ng/g. The method was validated for the analysis of real honey samples.

UI MeSH Term Description Entries
D008795 Metronidazole A nitroimidazole used to treat AMEBIASIS; VAGINITIS; TRICHOMONAS INFECTIONS; GIARDIASIS; ANAEROBIC BACTERIA; and TREPONEMAL INFECTIONS. 2-Methyl-5-nitroimidazole-1-ethanol,Bayer 5360,Clont,Danizol,Flagyl,Gineflavir,Metric,MetroGel,Metrodzhil,Metrogyl,Metronidazole Hydrochloride,Metronidazole Monohydrochloride,Metronidazole Phosphate,Metronidazole Phosphoester,Satric,Trichazol,Trichopol,Trivazol,Vagilen,2 Methyl 5 nitroimidazole 1 ethanol
D008956 Models, Chemical Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment. Chemical Models,Chemical Model,Model, Chemical
D009593 Nitroimidazoles IMIDAZOLES having a nitro moiety. Nitroimidazole
D002623 Chemistry Techniques, Analytical Methodologies used for the isolation, identification, detection, and quantitation of chemical substances. Analytical Chemistry Techniques,Analytical Chemistry Methods,Analytical Chemistry Method,Analytical Chemistry Technique,Chemistry Method, Analytical,Chemistry Methods, Analytical,Chemistry Technique, Analytical,Method, Analytical Chemistry,Methods, Analytical Chemistry,Technique, Analytical Chemistry,Techniques, Analytical Chemistry
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004131 Dimetridazole A compound used to treat histomoniasis in poultry. Emtryl
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004350 Drug Residues Drugs and their metabolites which are found in the edible tissues and milk of animals after their medication with specific drugs. This term can also apply to drugs found in adipose tissue of humans after drug treatment. Drug Residue,Residue, Drug,Residues, Drug
D005504 Food Analysis Measurement and evaluation of the components of substances to be taken as FOOD. Analysis, Food,Analyses, Food,Food Analyses
D006722 Honey A sweet viscous liquid food, produced in the honey sacs of various bees from nectar collected from flowers. The nectar is ripened into honey by inversion of its sucrose sugar into fructose and glucose. It is somewhat acidic and has mild antiseptic properties, being sometimes used in the treatment of burns and lacerations. Honeys

Related Publications

Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
January 2004, Veterinaria italiana,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
June 1997, Journal of chromatography. B, Biomedical sciences and applications,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
July 1993, Journal of chromatography,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
March 2013, Archives of pharmacal research,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
January 2012, Journal of AOAC International,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
January 2007, Journal of AOAC International,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
June 2016, Analytical biochemistry,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
May 2009, Vojnosanitetski pregled,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
December 2009, Journal of pharmaceutical and biomedical analysis,
Jinhui Zhou, and Jianzhong Shen, and Xiaofeng Xue, and Jing Zhao, and Yi Li, and Jinzhen Zhang, and Suxia Zhang
October 2017, Journal of separation science,
Copied contents to your clipboard!