BIOMAT Database Logo BIOMAT Database Logo

Antibodies from women immunized with Gardasil cross-neutralize HPV 45 pseudovirions. 2007

Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Vaccine and Biologics Research, MRL, Merck & Co., Inc., West Point, Pennsylvania, USA.

Human papillomavirus (HPV) types 6, 11, 16 and 18 L1 virus-like particles (VLPs) have been used to generate the prophylactic quadrivalent vaccine, Gardasil. There is a high degree of L1 homology between HPV types and it is likely that there is a substantial degree of surface exposed viral epitope similarity. An investigation of vaccine-induced antibody binding and neutralization was undertaken focusing on A7 species members, HPV 18 and 45. Polyclonal sera from Gardasil recipients and from HPV 18 L1 VLP recipients were evaluated. Vaccine-induced antibodies were found to cross-neutralize HPV 45 pseudovirions (PsV) in vitro. Examination of a panel of monoclonal antibodies made against L1 VLPs revealed the presence of conformational, neutralizing epitopes on the surface of VLPs that may be shared between HPV 18 and HPV 45. These data demonstrate that Gardasil(r) immunization induces antibodies capable of neutralizing HPV 18 PsV and HPV 45 PsV in vitro.

UI MeSH Term Description Entries
D007114 Immunization Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow). Immunologic Stimulation,Immunostimulation,Sensitization, Immunologic,Variolation,Immunologic Sensitization,Immunological Stimulation,Sensitization, Immunological,Stimulation, Immunologic,Immunizations,Immunological Sensitization,Immunological Sensitizations,Immunological Stimulations,Sensitizations, Immunological,Stimulation, Immunological,Stimulations, Immunological,Variolations
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000068857 Human Papillomavirus Recombinant Vaccine Quadrivalent, Types 6, 11, 16, 18 A vaccine containing L1 capsid proteins from four types of HPV (ALPHAPAPILLOMAVIRUS), types 6, 11, 16 and 18 that is used to prevent infections from human papillomaviruses of these subtypes. Human Papillomavirus Vaccine L1, Types 6,11,16,18,Gardasil,HPV L1 Vaccine, Quadrivalent 6,11,16,18,Human Papillomavirus Vaccine L1, Type 6,11,16,18
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D000917 Antibody Formation The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS. Antibody Production,Antibody Response,Antibody Responses,Formation, Antibody,Production, Antibody,Response, Antibody,Responses, Antibody

Related Publications

Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Characterization of serum antibodies from women immunized with Gardasil: A study of HPV-18 infection of primary human keratinocytes.
June 2016, Vaccine,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Serum antibodies to human papillomavirus (HPV) pseudovirions correlate with natural infection for 13 genital HPV types.
April 2013, Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Gardasil administration to hr-HPV-positive women and their partners.
September 2013, Trends in pharmacological sciences,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Papillomavirus pseudovirions packaged with the L2 gene induce cross-neutralizing antibodies.
March 2010, Journal of translational medicine,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
HPV pseudovirions as DNA delivery vehicles.
April 2011, Therapeutic delivery,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Detection of neutralizing antibodies against human papillomaviruses (HPV) by inhibition of gene transfer mediated by HPV pseudovirions.
March 2002, Journal of clinical microbiology,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Gardasil 9--a broader HPV vaccine.
March 2015, The Medical letter on drugs and therapeutics,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Function matters: Coronavirus cross-binding antibodies do not cross-neutralize.
January 2022, Frontiers in medicine,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
Neutralization of human papillomavirus (HPV) pseudovirions: a novel and efficient approach to detect and characterize HPV neutralizing antibodies.
December 2000, Virology,
Judith F Smith, and Michelle Brownlow, and Martha Brown, and Rose Kowalski, and Mark T Esser, and Wanda Ruiz, and Eliav Barr, and Darron R Brown, and Janine T Bryan
HPV vaccination with Gardasil: a breakthrough in women's health.
November 2006, Expert opinion on biological therapy,
Copied contents to your clipboard!