The manuscript describes removal of chromium from aqueous solution by biomass of different moulds and yeasts. The biomass of Termitomyces clypeatus (TCB) is found to be the most effective of all the fungal species tested. The sorption of hexavalent chromium by live TCB depends on the pH of the solution, the optimum pH value being 3.0. The process follows Langmuir isotherm (regression coefficient 0.998, chi(2)-square 5.03) model with uniform distribution over the surface which gets strong support from the X-ray elemental mapping of chromium adsorbed biomass. The amino, carboxyl, hydroxyl, and phosphate groups of the biomass are involved in chemical interaction with the chromate ion forming a cage like structure depicted by scanning electron microscopic (SEM) and Fourier transform infrared spectroscopic (FTIR) results. Desorption and FTIR studies also exhibited that Cr(6+) is reduced to trivalent chromium on binding to the cell surface. The level of chromium concentration present in the effluent of tannery industries' is reduced to a permissible limit using TCB as adsorbent.