-transparent.png){kind=logo}
Label-free optical detection and tracking of single virions bound to their receptors in supported membrane bilayers. 2007
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
Institute of Biochemistry, ETH Zurich, CH-8093 Zurich, Switzerland.
We apply an interferometric optical detection scheme to image and track unlabeled single virions. Individual simian virus 40 virions and uninfectious virus-like particles were imaged on a glass substrate and on a supported membrane bilayer. Moreover, single unlabeled virions were tracked when bound to supported membrane bilayers via the viral receptor, the glycolipid GM1. The technology presented here promises to be generally applicable to studying the motion of unlabeled macromolecules on membranes.
Related Publications
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
January 2019,
Methods in molecular biology (Clifton, N.J.),
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
January 2014,
Nano letters,
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
November 2010,
Analytical chemistry,
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
January 2013,
Methods in molecular biology (Clifton, N.J.),
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
August 2007,
Science (New York, N.Y.),
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
October 2021,
Nature methods,
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
January 2015,
Methods in cell biology,
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
December 2015,
Journal of visualized experiments : JoVE,
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
October 2018,
Analytical chemistry,
Helge Ewers, and
Volker Jacobsen, and
Enrico Klotzsch, and
Alicia E Smith, and
Ari Helenius, and
Vahid Sandoghdar
April 2022,
Nature communications,
