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The semisolid agar method for the selective growth of transformed cells was applied to investigations of Epstein-Barr virus (EBV) transformation. When 1 X 10(5) HUMAn umbilical-cord blood leukocytes were seeded in agar medium immediately after EBV exposure, about 100 colonies developed in each dish. The occurrence of colonies correlated well with dilutions of EBV inoculum. These colonies were composed of lymphoblasts, were positive for EBV-determined nuclear antigen immunofluorescence, and consistently resulted in the establishment of cell lines. In cord leukocyte cultures exposed to EBV, cells capable of growing in semisolid agar medium appeared early and increased rapidly, though the total numbers of leukocytes and control cultures decreased during the first several days.
F Mizuno, and
T Aya, and
T Osato
February 1976,
International journal of cancer,
F Mizuno, and
T Aya, and
T Osato
January 1973,
Proceedings of the National Academy of Sciences of the United States of America,
F Mizuno, and
T Aya, and
T Osato
January 1979,
Antimicrobial agents and chemotherapy,
F Mizuno, and
T Aya, and
T Osato
February 1975,
International journal of cancer,
F Mizuno, and
T Aya, and
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October 1978,
Journal of the National Cancer Institute,
F Mizuno, and
T Aya, and
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April 1984,
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F Mizuno, and
T Aya, and
T Osato
April 1974,
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F Mizuno, and
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January 2000,
Viral immunology,
F Mizuno, and
T Aya, and
T Osato
September 1980,
Infection and immunity,
F Mizuno, and
T Aya, and
T Osato
September 1977,
Journal of virology,
