-transparent.png){kind=logo}
Fluorescence of eight tryptophan residues in cytochrome P-450scc with bound endogenous cholesterol could be fitted with a two component model: a single exponential and a "top-hat" distribution of lifetimes as the second component. The short-lived component (tau 1 about 700 ps) does not change significantly upon binding of substrate (22R-hydroxycholesterol). The parameters of the long-lived component (central lifetime tau m about 3.4 ns) change upon binding of carbon monoxide and substrate. 22R-hydroxycholesterol binding broadens the distribution of the long-lived component; that is the heterogeneity of the Trp environment is increased when this substrate displaces the endogenous cholesterol.
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
February 1993,
Biochimica et biophysica acta,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
June 1999,
European journal of biochemistry,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
February 1982,
The Journal of biological chemistry,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
October 1994,
Molecular and cellular endocrinology,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
November 1994,
Molecular and cellular endocrinology,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
October 1982,
Proceedings of the National Academy of Sciences of the United States of America,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
January 1991,
FEBS letters,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
May 1982,
The Journal of biological chemistry,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
May 1993,
Biochimica et biophysica acta,
P Anzenbacher, and
J Hudecek, and
S Vajda, and
V Fidler, and
C Larroque, and
R Lange
September 1987,
Science (New York, N.Y.),
