-transparent.png){kind=logo}
Human polymorphonuclear leukocytes kill WEHI 164 cells in an 18-hour 51Cr release assay. Antibody to human tumor necrosis factor (TNF) blocks the lysis of targets mediated by human granulocytes. Resting granulocytes produce an undetectable amount of TNF, if any. Granulocytes stimulated with Staphylococcus aureus release 250-500 U/ml TNF alpha. The specificity of the released TNF in the WEHI 164 cytotoxicity assay was confirmed by using neutralizing anti-TNF alpha monoclonal antibodies. The thymidine uptake of endothelial cells was inhibited by granulocyte-derived TNF. The identity of TNF alpha was further confirmed by molecular weight determination, by gel filtration on Sephacryl S-200, with a result of approximately 44,000. Besides their antimicrobial capacity, therefore, granulocytes may contribute to tumor rejection, inflammation and septic infections by releasing TNF.
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
February 1987,
Blood,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
December 1986,
The American journal of pathology,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
March 1990,
Journal of leukocyte biology,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
September 1990,
Proceedings of the National Academy of Sciences of the United States of America,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
February 1985,
The Journal of biological chemistry,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
July 1989,
Cancer research,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
October 1991,
The Journal of infectious diseases,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
January 1991,
International archives of allergy and applied immunology,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
January 1990,
International journal of immunopharmacology,
Y Mándi, and
V Endrész, and
L Krenács, and
K Régely, and
M Degré, and
I Béládi
April 1998,
Journal of periodontology,
