Biomaterial Database

[Effect of parathyroid hormone and 1,25-(OH)2-D3 on the expression of the mRNA of calbindin D9k in Caco-2 cells]. 2007

Xuedan Mao, and Zebin Mao, and Zhuoping Huo

Institute of Nutrition and Food Safety, China Center for Disease Control and Prevention, Beijing 100021, China.

OBJECTIVE To study the effect of parathyroid hormone (PTH) on the expression of the mRNA of calbindin D9k(CaBP-D9k) in Caco-2 Cells and whether PTH influences the effect of 1,25-(OH)2-D3 on the expression of calbindin mRNA in Caco-2 Cells. METHODS Caco-2 cells were used as the model of human small intestinal enterocytes. Caco-2 cells were treated 5, 10, 20, 40 and 80 min at the dose of 10(-8), 10(-9) and 10(-12) mol/L PTH, another groups of Caco-2 cells were treated for 2, 4, 8, 16 and 24h at the dose of 10(-8) mol/L 1,25-(OH)2-D3 alone. The other groups of Caco-2 cells were treated for 2, 4, 8, 16 and 24h at the dose of 10(-8) mol/L 1,25-(OH)2-D3 plus at the dose of 10(-8), 10(-9) and 10(-12) mol/L of PTH respectively. 0.1% ethanol solutions were used as the vehicle for the later two groups. The expression of calbindin D9k mRNA was determined by RT-PCR (the level of GAPDH was taken as internal control). RESULTS The expressions of calbindin D9k mRNA were more higher than those of blank controls when Caco-2 cells were treated at the dose of 10(-8) mol/L 1,25-(OH)2-D3 for 2, 4, 8, 16 and 24h, the expressions of CaBP-D9k mRNA were higher than those of vehicle. The increases of CaBP-D9k mRNA levels caused by 10(-8) mol/L 1,25-(OH)2-D3 plus PTH were more lower than those caused by 1,25-(OH)2-D3 alone. CONCLUSIONS 10(-8) and 10(-12) mol/L PTH probably caused increase on the expression of CaBP-D9k mRNA in short period (1 - 20min). 10(-8) mol/L 1,25-(OH)2-D3 increases CaBP-D9k mRNA levels in Caco-2 cells. PTH may inhibit or counteract the increase in CaBP-D9k mRNA caused by 1,25-(OH)2-D3 in Caco-2 cells.

UI	MeSH Term	Description	Entries
D010281	Parathyroid Hormone	A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.	Natpara,PTH (1-84),PTH(1-34),Parathormone,Parathyrin,Parathyroid Hormone (1-34),Parathyroid Hormone (1-84),Parathyroid Hormone Peptide (1-34),Hormone, Parathyroid
D002117	Calcitriol	The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.	1 alpha,25-Dihydroxycholecalciferol,1 alpha,25-Dihydroxyvitamin D3,1, 25-(OH)2D3,1,25(OH)2D3,1,25-Dihydroxycholecalciferol,1,25-Dihydroxyvitamin D3,1 alpha, 25-dihydroxy-20-epi-Vitamin D3,1,25(OH)2-20epi-D3,1,25-dihydroxy-20-epi-Vitamin D3,20-epi-1alpha,25-dihydroxycholecaliferol,Bocatriol,Calcijex,Calcitriol KyraMed,Calcitriol-Nefro,Decostriol,MC-1288,MC1288,Osteotriol,Renatriol,Rocaltrol,Silkis,Sitriol,Soltriol,Tirocal,1 alpha,25 Dihydroxyvitamin D3,1,25 Dihydroxycholecalciferol,1,25 Dihydroxyvitamin D3,1,25 dihydroxy 20 epi Vitamin D3,Calcitriol Nefro,D3, 1 alpha,25-Dihydroxyvitamin,D3, 1,25-Dihydroxyvitamin,D3, 1,25-dihydroxy-20-epi-Vitamin,KyraMed, Calcitriol,MC 1288
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012333	RNA, Messenger	RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.	Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D018938	Caco-2 Cells	Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.	Caco 2 Cells,Caco-2 Cell,Cell, Caco-2,Cells, Caco-2
D064026	Calbindins	Calcium-binding proteins that are found in DISTAL KIDNEY TUBULES, INTESTINES, BRAIN, and other tissues where they bind, buffer and transport cytoplasmic calcium. Calbindins possess a variable number of EF-HAND MOTIFS which contain calcium-binding sites. Some isoforms are regulated by VITAMIN D.	Calbindin
D064030	S100 Calcium Binding Protein G	A calbindin protein found in many mammalian tissues, including the UTERUS, PLACENTA, BONE, PITUITARY GLAND, and KIDNEYS. In intestinal ENTEROCYTES it mediates intracellular calcium transport from apical to basolateral membranes via calcium binding at two EF-HAND MOTIFS. Expression is regulated in some tissues by VITAMIN D.	Calbindin 3,Calbindin D9K,Calbindin-D9K,Calcium Binding Protein, Vitamin D Dependent,Calcium-Binding Protein, Vitamin D-Dependent,Cholecalcin,IMCal Protein,Intestinal Membrane Calcium-Binding Protein,Vitamin D-Dependent Calcium-Binding Protein,Intestinal Membrane Calcium Binding Protein,Vitamin D Dependent Calcium Binding Protein