In vitro assessment of gastrointestinal viability of two photosynthetic bacteria, Rhodopseudomonas palustris and Rhodobacter sphaeroides. 2007

Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310027, China.

The objectives of this study were to assess the potential of two photosynthetic bacteria (PSB), Rhodopseudomonas palustris HZ0301 and Rhodobacter sphaeroides HZ0302, as probiotics in aquaculture. The viability of HZ0301 and HZ0302 in simulated gastric transit conditions (pH 2.0, pH 3.0 and pH 4.0 gastric juices) and in simulated small intestinal transit conditions (pH 8.0, with or without 0.3% bile salts) was tested. The effects of HZ0301 and HZ0302 on the viability and permeability of intestinal epithelial cell in primary culture of tilapias, Oreochromis nilotica, were also detected. All the treatments were determined with three replicates. The simulated gastric transit tolerance of HZ0301 and HZ0302 strains was pH-dependent and correspondingly showed lower viability at pH 2.0 after 180 min compared with pH 3.0 and pH 4.0. Both HZ0301 and HZ0302 were tolerant to simulated small intestine transit with or without bile salts in our research. Moreover, there was no significant difference (P>0.05) among three treatments including the control and the groups treated with HZ0301 or HZ0302 both in intestinal epithelial cell viability and membrane permeability, showing no cell damage. In summary, this study demonstrated that HZ0301 and HZ0302 had high capacity of upper gastrointestinal transit tolerance and were relatively safe for intestinal epithelial cells of tilapias.

UI MeSH Term Description Entries
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012241 Rhodopseudomonas A genus of gram-negative, rod-shaped, phototrophic bacteria found in aquatic environments. Internal photosynthetic membranes are present as lamellae underlying the cytoplasmic membrane.
D012242 Rhodobacter sphaeroides Spherical phototrophic bacteria found in mud and stagnant water exposed to light. Rhodopseudomonas sphaeroides,Rhodobacter spheroides,Rhodopseudomonas spheroides
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D017210 Tilapia A freshwater fish used as an experimental organism and for food. This genus of the family Cichlidae (CICHLIDS) inhabits Central and South America (one species extends north into Texas), West Indies, Africa, Madagascar, Syria, and coastal India. Oreochromis mossambicus,Tilapia mossambica,Mozambique Tilapia,Mozambique Tilapias,Tilapia, Mozambique,Tilapias, Mozambique
D041981 Gastrointestinal Tract Generally refers to the digestive structures stretching from the MOUTH to ANUS, but does not include the accessory glandular organs (LIVER; BILIARY TRACT; PANCREAS). Digestive Tract,GI Tract,Digestive Tracts,GI Tracts,Gastrointestinal Tracts
D050296 Microbial Viability Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate. Bacterial Viability,Virus Viability,Bacteria Viability,Microbial Inactivation,Inactivation, Microbial,Viability, Bacteria,Viability, Bacterial,Viability, Microbial,Viability, Virus
D052817 Phototrophic Processes Processes by which phototrophic organisms use sunlight as their primary energy source. Contrasts with chemotrophic processes which do not depend on light and function in deriving energy from exogenous chemical sources. Photoautotrophy (or photolithotrophy) is the ability to use sunlight as energy to fix inorganic nutrients to be used for other organic requirements. Photoautotrophs include all GREEN PLANTS; GREEN ALGAE; CYANOBACTERIA; and green and PURPLE SULFUR BACTERIA. Photoheterotrophs or photoorganotrophs require a supply of organic nutrients for their organic requirements but use sunlight as their primary energy source; examples include certain PURPLE NONSULFUR BACTERIA. Depending on environmental conditions some organisms can switch between different nutritional modes (AUTOTROPHY; HETEROTROPHY; chemotrophy; or phototrophy) to utilize different sources to meet their nutrients and energy requirements. Phototrophic Growth,Phototrophic Process,Phototrophy,Growth, Phototrophic,Process, Phototrophic,Processes, Phototrophic

Related Publications

Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
June 2004, Microbiology (Reading, England),
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
January 2008, Izvestiia Akademii nauk. Seriia biologicheskaia,
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
January 1967, Archiv fur Mikrobiologie,
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
August 1991, Biochimica et biophysica acta,
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
November 2012, Wei sheng yan jiu = Journal of hygiene research,
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
April 2005, Biotechnology letters,
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
April 2009, Colloids and surfaces. B, Biointerfaces,
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
April 2007, Proceedings of the National Academy of Sciences of the United States of America,
Xu-xia Zhou, and Yuan-jiang Pan, and Yan-bo Wang, and Wei-fen Li
July 2009, The journal of physical chemistry. B,
Copied contents to your clipboard!