-transparent.png){kind=logo}
IN vitro peroxidation by air, or xanthine-xanthine oxidase (xanthine-XOD) was performed to estimate the production of aliphatic aldehydes from free polyunsaturated fatty acids (PUFA), triglycerides, phospholipids and rat liver microsomes and mitochondria. The aldehyde contents in peroxidized lipids were determined by liquid chromatography and fluorescence detection. In both peroxidation, pentanal, (E)-4-hydroxy-2-nonenal (4-HN), and hexanal were produced from omega-6 PUFA rich lipids and propanal was markedly enhanced by increasing the degree of fatty acid unsaturation. The ratios of 4-HN to hexanal production in xanthine-XOD peroxidation of the omega-6 PUFA rich lipids, and rat liver microsomes and mitochondria were much higher than those in air peroxidation. The ratios (4-HN/hexanal) obtained in microsomes and mitochondria by xanthine-XOD were similar to those in rat liver observed in vitamin E deficient studies. The determination of these aldehydes may be useful to estimate the kinds of fatty acids peroxidized and investigate in vivo lipid peroxidation mechanism.
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
January 1997,
Journal of bioluminescence and chemiluminescence,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
January 1947,
Federation proceedings,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
January 1969,
Physiologia plantarum,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
January 2013,
Journal of the American Chemical Society,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
November 1952,
Comptes rendus hebdomadaires des seances de l'Academie des sciences,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
January 1976,
Biochimie,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
March 1946,
Food materials and equipment,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
February 1984,
Biochimica et biophysica acta,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
May 2023,
Journal of agricultural and food chemistry,
K Yoshino, and
M Sano, and
M Jujita, and
I Tomita
January 2013,
Bioscience, biotechnology, and biochemistry,
