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A simple method is described for producing avirulent mouse brain antigens for complement-fixation tests in human encephalitides, by application of heat at 60 degrees C for 30 minutes. The heated antigens retain for most of the viruses employed a sufficiently high titer to warrant their use, since they are also specific, not anticomplementary, can be lyophilized and moreover, are avirulent, and thus can be handled with safety.
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