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Bovine serum panel for evaluating foot-and-mouth disease virus nonstructural protein antibody tests. 2007
Satya Parida, and
Lucy Fleming, and
Debi Gibson, and
Pip A Hamblin, and
Santina Grazioli, and
Emiliana Brocchi, and
David J Paton
Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination. Virus replication and persistence had been characterized in all of the animals. The proportion of the sera scored positive by 5 tests for antibodies to the nonstructural proteins of FMDV varied, suggesting that the panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of this panel will help in assessment of new tests and quality control of existing methods.
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