Detection of the disease-associated isoform of the prion protein in formalin-fixed tissues by Western blot. 2007

Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA. eric.nicholson@ars.usda.gov

Clinical signs of prion disease are not specific and include a variety of differential diagnoses. Serological tests and nucleic acid-based detection methods are not applicable to prion-disease-agent detection because of the unusual nature of the infectious agent. Prion-disease diagnosis is primarily conducted by means of immunodetection of the infectious agent, typically by at least 2 distinct procedures with immunohistochemistry and Western blot being the most informative. These approaches differ in the need for formalin-fixed and frozen or fresh tissue respectively. This work describes a method for the detection of the disease-associated isoform of the prion protein by Western blot using formalin-fixed tissues. The approach requires only minimal modification of existing Western-blot procedures and could readily be incorporated into existing detection schemes for confirmatory purposes when fresh or frozen tissues are unavailable.

UI MeSH Term Description Entries
D001933 Brain Stem The part of the brain that connects the CEREBRAL HEMISPHERES with the SPINAL CORD. It consists of the MESENCEPHALON; PONS; and MEDULLA OBLONGATA. Brainstem,Truncus Cerebri,Brain Stems,Brainstems,Cerebri, Truncus,Cerebrus, Truncus,Truncus Cerebrus
D005557 Formaldehyde A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717) Formalin,Formol,Methanal,Oxomethane
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012608 Scrapie A fatal disease of the nervous system in sheep and goats, characterized by pruritus, debility, and locomotor incoordination. It is caused by proteinaceous infectious particles called PRIONS. Rida
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D016707 Tissue Fixation The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements. Fixation, Tissue
D018620 PrPSc Proteins Abnormal isoform of PRION PROTEINS resulting from a posttranslational modification of the cellular prion protein (PRPC PROTEINS). PrPSc are disease-specific proteins seen in certain human and animal neurodegenerative diseases (PRION DISEASES). Scrapie Agent,Scrapie PrP,HaSp 33-37,PrP (CJD),PrP (GSS),PrP 33-35,PrP-res,Scrapie HaSp33-37 Protein,Scrapie PrP 33-35,Scrapie Virus,Sp 33-35,PrP res
D020033 Protein Isoforms Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING. Isoform,Isoforms,Protein Isoform,Protein Splice Variant,Splice Variants, Protein,Protein Splice Variants,Isoform, Protein,Isoforms, Protein,Splice Variant, Protein,Variant, Protein Splice,Variants, Protein Splice

Related Publications

Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
July 2011, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
November 1998, Journal of virological methods,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
January 1986, Histochemistry,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
May 2010, Journal of virological methods,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
July 1994, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
May 1997, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
January 2001, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
September 2010, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
April 2010, Analytical biochemistry,
Eric M Nicholson, and Robert A Kunkle, and Amir N Hamir, and Semakaleng Lebepe-Mazur, and Dennis Orcutt
January 2020, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!