BIOMAT Database Logo BIOMAT Database Logo

DNA probes and the detection of food-borne pathogens using the polymerase chain reaction. 1991

R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
A.F.R.C., Institute of Food Research, Norwich Research Park, U.K.

UI MeSH Term Description Entries
D008087 Listeria A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.
D008089 Listeria monocytogenes A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001419 Bacteria One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive. Eubacteria
D015342 DNA Probes Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. Chromosomal Probes,DNA Hybridization Probe,DNA Probe,Gene Probes, DNA,Conserved Gene Probes,DNA Hybridization Probes,Whole Chromosomal Probes,Whole Genomic DNA Probes,Chromosomal Probes, Whole,DNA Gene Probes,Gene Probes, Conserved,Hybridization Probe, DNA,Hybridization Probes, DNA,Probe, DNA,Probe, DNA Hybridization,Probes, Chromosomal,Probes, Conserved Gene,Probes, DNA,Probes, DNA Gene,Probes, DNA Hybridization,Probes, Whole Chromosomal
D015698 Genomic Library A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns). Genome Library,Genome Libraries,Genomic Libraries,Libraries, Genome,Libraries, Genomic,Library, Genome,Library, Genomic
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain

Related Publications

R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Probes and polymerase chain reaction for detection of food-borne bacterial pathogens.
November 1995, International journal of food microbiology,
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Detection of Chlamydia psittaci using DNA probes and the polymerase chain reaction.
January 1991, FEMS microbiology letters,
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Detection of tick-borne pathogens by MassTag polymerase chain reaction.
April 2009, Vector borne and zoonotic diseases (Larchmont, N.Y.),
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Quantitative detection of periodontal pathogens using real-time polymerase chain reaction with TaqMan probes.
June 2004, Oral microbiology and immunology,
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
[Detection of Mycoplasma pneumoniae by using polymerase chain reaction and nonradioactive DNA probes].
December 1995, Rinsho byori. The Japanese journal of clinical pathology,
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Detection of untreated mycobacteria by using polymerase chain reaction and specific DNA probes.
August 1991, Journal of clinical microbiology,
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
A subtractively optimized DNA microarray using non-sequenced genomic probes for the detection of food-borne pathogens.
May 2011, Applied biochemistry and biotechnology,
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Detection of animal pathogens by using the polymerase chain reaction (PCR).
May 1997, Veterinary journal (London, England : 1997),
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Microarray detection of food-borne pathogens using specific probes prepared by comparative genomics.
October 2008, Biosensors & bioelectronics,
R A McKee, and C M Gooding, and S D Garrett, and H A Powell, and B M Lund, and M Knox
Labeling of DNA Probes by Polymerase Chain Reaction.
March 2022, Cold Spring Harbor protocols,
Copied contents to your clipboard!