Rapid cultivation of Mycobacterium tuberculosis in Veeraraghavan's liquid medium. 1991

F K Daginawala, and D D Banker
Sir Hurkisondas Nurrotumdas Hospital, Bombay.

2015 clinical samples were screened for M. tuberculosis culture in Veeraraghavan's liquid medium V (T). The liquid medium is a synthetic medium rich in amino acids, vitamins, sugars and mineral salts. Initially 111 consecutive samples of sputum and various body fluids were cultured in both LJ medium and liquid synthetic medium for comparison. As liquid medium gave satisfactory and quicker results the subsequent 1904 samples were cultured only on liquid medium to determine its usefulness in routine work. A total of 215 of the 243 ZN smear positive samples successfully grew within 48 hours in the liquid culture medium. Drug sensitivity was performed on the positive cultures at 10, 20, 40 and 80 micrograms/ml concentrations in the liquid medium with RNA. The sensitivity results were obtained within next 48 hours. The sensitivity tests on the cultures under study revealed highest sensitivity to Rifampicin and lowest to Isoniazid. Identification and pigment production of the mycobacterial culture was also done successfully in Veeraraghavan's liquid medium. The liquid medium is cost-effective and is recommended for routine use.

UI MeSH Term Description Entries
D009169 Mycobacterium tuberculosis A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation. Mycobacterium tuberculosis H37Rv
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic

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