-transparent.png){kind=logo}
BACKGROUND Dendritic cells are key contributors to initiation and maintenance of T-cell immunity to inhaled allergen. The purpose of this study was to enumerate the changes in peripheral blood myeloid (mDCs) and plasmacytoid dendritic cells (pDCs), the DCs expressing chemokine receptor 6 (CCR6) and chemokine receptor 7 (CCR7), following diluent and allergen inhalation in asthmatic subjects. METHODS Peripheral blood was obtained from 16 allergic asthmatic subjects before and at 0.5, 1, 2, 3, 4, 6, 24, and 48 h after inhaled diluent and allergen challenges. Dendritic cells were enumerated using flow cytometry. RESULTS Allergen inhalation significantly reduced mDCs at 6 h (21.3 +/- 2.0 vs 15.0 +/- 1.8/microl blood; P < 0.05) and 24 h (21.5 +/- 3.4 vs 16.4 +/- 2.4/microl blood; P < 0.05) after challenge. Circulating pDCs were significantly lower than baseline up to 24 h after both allergen and diluent challenges. There was a significant efflux of CCR6(+) mDCs from peripheral blood at 6 h and CCR6(+) pDCs at 4 h after allergen challenge, when compared with diluent. There was no difference in the number of circulating CCR7(+) mDCs or pDCs after diluent or allergen challenges. CONCLUSIONS Peripheral blood mDCs and CCR6(+) mDCs, but not pDCs, are reduced up to 24 h after allergen inhalation. Thus, allergen inhalation causes trafficking of immature CCR6(+) DCs from blood into the airway, while that of the trafficking of the mature CCR7(+) DCs from the airways into the regional lymph nodes probably occurs through the lymphatic system.
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
July 2014,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
July 2010,
The Journal of allergy and clinical immunology,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
June 2002,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
July 1989,
Thorax,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
March 2019,
Journal of breast cancer,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
December 2021,
International immunopharmacology,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
June 2003,
The Journal of pathology,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
August 2011,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
August 2005,
The Journal of infectious diseases,
E Farrell, and
T M O'Connor, and
M Duong, and
R M Watson, and
T Strinich, and
G M Gauvreau, and
P M O'Byrne
January 2014,
BioMed research international,
