Immunolocalization of muscarinic receptor subtypes in the reticular thalamic nucleus of rats. 2007

Satoko Oda, and Fumi Sato, and Akiko Okada, and Satomi Akahane, and Hiroaki Igarashi, and Junko Yokofujita, and Junli Yang, and Masaru Kuroda
Department of Anatomy, Toho University School of Medicine, Tokyo, Japan. odas@med.toho-u.ac.jp

In this study, to identify the precise localization of the muscarinic receptor subtypes m2, m3 and m4 in the rostral part of the rat reticular thalamic nucleus (rRt), namely, the limbic sector, we used receptor-subtype-specific antibodies and characterized the immunolabeled structures by light, confocal laser scanning, and electron microscopies. The m2-immunolabeling was preferentially distributed in the distal dendrite region where cholinergic afferent fibers tend to terminate and in the peripheral region of somata, whereas the m3-immunolabeling was more preferentially distributed in a large part of somata and in proximal dendrite shafts than in the distal dendrite region. Dual-immunofluorescence experiments demonstrated that majority of rRt neurons with parvalbumin immunoreactivity contain both m2 and m3. Neither m2 nor m3 was detected in presynaptic terminals or axonal elements. No m4-immunolabeling was detected in the rostral part of the thalamus including rRt. These results show the different distributions of m2 and m3 in rRt neurons, and strongly suggest that m2 is more closely associated with cholinergic afferents than m3.

UI MeSH Term Description Entries
D008297 Male Males
D008959 Models, Neurological Theoretical representations that simulate the behavior or activity of the neurological system, processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment. Neurologic Models,Model, Neurological,Neurologic Model,Neurological Model,Neurological Models,Model, Neurologic,Models, Neurologic
D010320 Parvalbumins Low molecular weight, calcium binding muscle proteins. Their physiological function is possibly related to the contractile process. Parvalbumin,Parvalbumin B
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013787 Thalamic Nuclei Several groups of nuclei in the thalamus that serve as the major relay centers for sensory impulses in the brain. Nuclei, Thalamic
D016253 Microscopy, Immunoelectron Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays. Immunoelectron Microscopy,Microscopy, Immuno-Electron,Immuno-Electron Microscopies,Immuno-Electron Microscopy,Immunoelectron Microscopies,Microscopies, Immuno-Electron,Microscopies, Immunoelectron,Microscopy, Immuno Electron
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D043585 Receptor, Muscarinic M2 A specific subtype of muscarinic receptor found in the lower BRAIN, the HEART and in SMOOTH MUSCLE-containing organs. Although present in smooth muscle the M2 muscarinic receptor appears not to be involved in contractile responses. Muscarinic Receptor M2,Muscarinic Receptors M2,Receptors, Muscarinic M2,M2 Receptor, Muscarinic,M2 Receptors, Muscarinic,M2, Muscarinic Receptor,M2, Muscarinic Receptors,Muscarinic M2 Receptor,Muscarinic M2 Receptors,Receptor M2, Muscarinic,Receptors M2, Muscarinic
D043587 Receptor, Muscarinic M3 A subclass of muscarinic receptor that mediates cholinergic-induced contraction in a variety of SMOOTH MUSCLES. Muscarinic Receptor M3,Muscarinic Receptors M3,Receptors, Muscarinic M3,M3 Receptor, Muscarinic,M3 Receptors, Muscarinic,M3, Muscarinic Receptor,M3, Muscarinic Receptors,Muscarinic M3 Receptor,Muscarinic M3 Receptors,Receptor M3, Muscarinic,Receptors M3, Muscarinic
D043588 Receptor, Muscarinic M4 A specific subtype of muscarinic receptor found in the CORPUS STRIATUM and the LUNG. It has similar receptor binding specificities to MUSCARINIC RECEPTOR M1 and MUSCARINIC RECEPTOR M2. Muscarinic Receptor M4,Muscarinic Receptors M4,Receptors, Muscarinic M4,M4 Receptor, Muscarinic,M4 Receptors, Muscarinic,M4, Muscarinic Receptor,M4, Muscarinic Receptors,Muscarinic M4 Receptor,Muscarinic M4 Receptors,Receptor M4, Muscarinic,Receptors M4, Muscarinic

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