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We studied the solubility properties of brain acetylated alpha-tubulin, as well as the localization of this tubulin in brain tissue. Endogenous unpolymerized tubulin and cytoskeletal tubulin were fractionated after brain Triton-solubilization. Using the immunoblotting technique, we found that acetylated alpha-tubulin was recovered in the cytoskeletal fraction, and that most (92%) of the acetylated microtubules of this fraction were depolymerized by cold/Ca2+ treatment. In another set of experiments, axonal and soma-dendritic preparations were found to have equivalent amounts of acetylated alpha-tubulin. By immunogold electron microscopy, we established that acetylated microtubules are widely distributed in dendrites of the central nervous system.
M Morales, and
E Fifková
January 1993,
Memorias do Instituto Oswaldo Cruz,
M Morales, and
E Fifková
February 1987,
The Journal of cell biology,
M Morales, and
E Fifková
October 1988,
Cell structure and function,
M Morales, and
E Fifková
October 1995,
Acta histochemica,
M Morales, and
E Fifková
January 1988,
Cell motility and the cytoskeleton,
M Morales, and
E Fifková
September 1992,
Cell biology international reports,
M Morales, and
E Fifková
January 1991,
Methods in enzymology,
M Morales, and
E Fifková
April 2010,
Molecular reproduction and development,
M Morales, and
E Fifková
June 2004,
Journal of experimental zoology. Part A, Comparative experimental biology,
M Morales, and
E Fifková
March 1999,
Journal of neurochemistry,
