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[Short-term assay of colon carcinogen--induction of micronuclei and apoptosis by dimethylhydrazine in the mouse colon crypt cells]. 1991

Q F Zhu
Henan Medical University, Zhengzhou.

The frequencies of micronuclei and apoptosis in the colon crypt cells of mice treated with colon carcinogen were studied. Two strains of mice, inbred C57BL and close colony Kun-ming mice were used in this experiment. The mice were killed at 24 hr after intraperitoneal injection of dimethylhydrazine (DMH) at doses of 10, 20 and 40 mg/kg body weight. The results indicated that the frequencies of micronuclei and apoptosis in the colon crypt cells of both C57BL and Kun-ming mice were positively correlated quantitatively with the doses of DMH. Our results are similar to that of Heddle obtained in C57BL mice. We propose that the assay of micronuclei and apoptosis in mouse colon crypt cells might be a rapid and sensitive test and useful as a screening method for potential colon carcinogens. Kun-ming mice may be used as the test animal for detecting nuclear anomalies in this assay in lieu of C57BL mice.

UI MeSH Term Description Entries
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D003106 Colon The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON. Appendix Epiploica,Taenia Coli,Omental Appendices,Omental Appendix,Appendices, Omental,Appendix, Omental
D004127 Dimethylhydrazines Hydrazines substituted with two methyl groups in any position. Dimethylhydrazine
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015197 Carcinogenicity Tests Tests to experimentally measure the tumor-producing/cancer cell-producing potency of an agent by administering the agent (e.g., benzanthracenes) and observing the quantity of tumors or the cell transformation developed over a given period of time. The carcinogenicity value is usually measured as milligrams of agent administered per tumor developed. Though this test differs from the DNA-repair and bacterial microsome MUTAGENICITY TESTS, researchers often attempt to correlate the finding of carcinogenicity values and mutagenicity values. Tumorigenicity Tests,Carcinogen Tests,Carcinogenesis Tests,Carcinogenic Activity Tests,Carcinogenic Potency Tests,Carcinogen Test,Carcinogenesis Test,Carcinogenic Activity Test,Carcinogenic Potency Test,Carcinogenicity Test,Potency Test, Carcinogenic,Potency Tests, Carcinogenic,Test, Carcinogen,Test, Carcinogenesis,Test, Carcinogenic Activity,Test, Carcinogenic Potency,Test, Carcinogenicity,Test, Tumorigenicity,Tests, Carcinogen,Tests, Carcinogenesis,Tests, Carcinogenic Activity,Tests, Carcinogenic Potency,Tests, Carcinogenicity,Tests, Tumorigenicity,Tumorigenicity Test
D048629 Micronuclei, Chromosome-Defective Defective nuclei produced during the TELOPHASE of MITOSIS or MEIOSIS by lagging CHROMOSOMES or chromosome fragments derived from spontaneous or experimentally induced chromosomal structural changes. Chromosome-Defective Micronuclei,Genotoxicant-Induced Micronuclei,Micronuclei, Genotoxicant-Induced,Micronucleus, Chromosome-Defective,Chromosome Defective Micronuclei,Chromosome-Defective Micronucleus,Genotoxicant Induced Micronuclei,Genotoxicant-Induced Micronucleus,Micronuclei, Chromosome Defective,Micronuclei, Genotoxicant Induced,Micronucleus, Chromosome Defective,Micronucleus, Genotoxicant-Induced

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