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1. We have investigated whether phospholipase C (PLC) might act on phospholipids in the nuclear membrane of rat liver, resulting in inhibition of nuclear T3 binding (INB activity). 2. Incubation of intact nuclei with PLC caused a time- and dose-dependent increase of INB activity; this was correlated with a rise of the free fatty acid concentration in the nuclear ether extract. 3. Removal of the nuclear membrane resulted in a loss of INB activity of the nuclei. 4. Other compounds liberated by the action of PLC (such as diacylglycerols, IP3 and phosphoalcohols), had no INB activity. 5. We conclude that PLC can liberate fatty acids from the rat liver-nuclear membrane via further degradation of the direct product diacylglycerol. 6. These fatty acids display inhibition of nuclear T3 binding in vitro.
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
November 1990,
Biochimica et biophysica acta,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
October 1988,
Metabolism: clinical and experimental,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
February 1980,
Journal of neurochemistry,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
July 1992,
Metabolism: clinical and experimental,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
January 1990,
The International journal of biochemistry,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
January 1985,
Journal of free radicals in biology & medicine,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
April 1971,
Biochimica et biophysica acta,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
March 1989,
FEBS letters,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
June 1992,
Biochimica et biophysica acta,
F R van Der Klis, and
A A Nijenhuis, and
W M Wiersinga
September 1991,
Biochemical and biophysical research communications,
