Biomaterial Database

Determination of glycyrrhizin in liqueurs by on-line coupled capillary isotachophoresis with capillary zone electrophoresis. 2007

Frantisek Kvasnicka, and Michal Voldrich, and Josef Vyhnálek

Department of Food Preservation and Meat Technology, Institute of Chemical Technology Prague, Technická 3, 166 28 Prague 6, Czech Republic. kvasnicf@vscht.cz

An on-line coupled capillary isotachophoresis-capillary zone electrophoresis method for the determination of glycyrrhizin in liqueurs is described. The optimised electrolyte system was 5 mM HCl+11 mM epsilon-aminocaproic acid+0.05% hydroxyethylcellulose+30% methanol (leading electrolyte), 5 mM caproic acid+30% methanol (terminating electrolyte) and 20 mM caproic acid+10 mM histidine+0.1% hydroxyethylcellulose+30% methanol (background electrolyte). Method characteristics, i.e., linearity (20-500 ng/ml), accuracy (recovery 99+/-4%), intra-assay repeatability (2%), intermediate repeatability (3.8%) and detection limit (8 ng/ml) were determined. Speed of analysis, low laboriousness, high sensitivity and low-running cost are the typical attributes of the capillary isotachophoresis-capillary zone electrophoresis method. Developed method was successfully applied to analysis of liqueurs with liquorice extract and some foods (sweets and food supplements) containing liquorice. Found levels of glycyrrhizin in liqueurs, sweets and food supplements varied between 1-16 mg/l, 850-1050 mg/kg and 1.6-1.8 g/kg, respectively.

UI	MeSH Term	Description	Entries
D009862	Online Systems	Computer-based Information systems having real-time remote access to information or processes.	On-Line Systems,On Line Systems,On-Line System,Online System,System, On-Line,System, Online,Systems, On-Line,Systems, Online
D012015	Reference Standards	A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.	Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D002138	Calibration	Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.	Calibrations
D003217	Conductometry	Determination of the quantity of a material present in a mixture by measurement of its effect on the electrical conductivity of the mixture. (Webster, 3d ed)	Titration, Conductometric,Conductometric Titration,Conductometric Titrations,Titrations, Conductometric
D004586	Electrophoresis	An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.	Electrophoreses
D005240	Feasibility Studies	Studies to determine the advantages or disadvantages, practicability, or capability of accomplishing a projected plan, study, or project.	Feasibility Study,Studies, Feasibility,Study, Feasibility
D005504	Food Analysis	Measurement and evaluation of the components of substances to be taken as FOOD.	Analysis, Food,Analyses, Food,Food Analyses
D006035	Glycyrrhiza	A genus of leguminous herbs or shrubs whose roots yield GLYCYRRHETINIC ACID and its derivative, CARBENOXOLONE.	Licorice,Glycyrrhiza glabra,Liquorice,Licorices,Liquorices
D000434	Alcoholic Beverages	Drinkable liquids containing ETHANOL.	Alcoholic Beverage,Beverage, Alcoholic,Beverages, Alcoholic
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity