Biomaterial Database

Heme oxygenase-1 induction by hemin protects liver cells from ischemia/reperfusion injury in cirrhotic rats. 2007

Hui Xue, and Hua Guo, and Ying-Chao Li, and Zhi-Ming Hao

Department of Gastroenterology, The First Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an 710061, Shaanxi Province, China. zj2@mail.xjtu.edu.cn

OBJECTIVE To investigate the potential protective effect of HO-1 on cirrhotic liver cells in rats. METHODS Male Wistar rats included in the current study were randomly divided into 5 groups as follows: normal (N) group; liver cirrhotic (LC) group; sham (S) group; I/R group and I/R + hemin group. The model for inducing liver cirrhosis in rats was established according to a previously published protocol. Following this the segmental hepatic ischemia reperfusion operation was carried out. The rats were treated with 30 micromol/kg hemin (HO-1 inducer, ferric portoporphyrin IX chloride) i.p. or 0.9% NaCl (control) 24 h and 12 h before hepatic ischemia for 30 min or sham laparotomy. Blood was collected for serum enzymatic measurement 6 and 12 h after reperfusion or sham laparotomy. HO-1, NF-kappaB and caspase-3 expressions were assessed by immunohistochemical analysis. RESULTS The expressions of proteins are inversely correlated to the gray values. HO-1 expression in the I/R + hemin group was increased significantly than I/R group at 6 h and 12 h after hepatic I/R (6 h: 112.0 +/- 8.3 vs 125.1 +/- 5.7, P < 0.01; 12 h: 120.8 +/- 11.0 vs 132.4 +/- 6.2, P < 0.01). Hemin improved serum manganese superoxide dismutase (MnSOD) (6 h: 131.3 +/- 17.6 vs 107.0 +/- 13.9, P < 0.01; 12 h: 141.4 +/- 12.5 vs 118.3 +/- 10.2, P < 0.01), lessened liver cell injury, decreased caspase-3(6 h: 166.7 +/- 8.1 vs 145.5 +/- 14.6, P < 0.01; 12 h: 172.8 +/- 3.8 vs 148.0 +/- 6.5, P < 0.01) and NF-kappaB expression (6 h: 150.2 +/- 8.6 vs 139.7 +/- 6.0, P < 0.01; 12 h: 151.1 +/- 5.9 vs 148.1 +/- 5.3, P > 0.05) and serum alanine aminotransferase (ALT) (6 h: 413.3 +/- 104.1 vs 626.8 +/- 208.2, P < 0.01; 12 h: 322.2 +/- 98.8 vs 425.8 +/- 115.4, P < 0.05), aspartate aminotransferase (AST) (6 h: 665.2 +/- 70.1 vs 864.3 +/- 70.4, P < 0.01; 12 h: 531.1 +/- 98.6 vs 664.4 +/- 115.6, P < 0.01), malondialdehyde (MDA) levels (6 h: 11.1 +/- 2.17 vs 13.5 +/- 2.01, P < 0.01; 12 h: 9.36 +/- 1.10 vs 10.8 +/- 1.62, P < 0.05) in the I/R + hemin group when compared with the I/R group. CONCLUSIONS These results suggest that HO-1 plays an important role in protecting liver cells from hepatic I/R injury in cirrhotic rats by decreasing oxidative stress, apoptosis and inflammation.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008103	Liver Cirrhosis	Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.	Cirrhosis, Liver,Fibrosis, Liver,Hepatic Cirrhosis,Liver Fibrosis,Cirrhosis, Hepatic
D008297	Male		Males
D011897	Random Allocation	A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.	Randomization,Allocation, Random
D002251	Carbon Tetrachloride	A solvent for oils, fats, lacquers, varnishes, rubber waxes, and resins, and a starting material in the manufacturing of organic compounds. Poisoning by inhalation, ingestion or skin absorption is possible and may be fatal. (Merck Index, 11th ed)	Tetrachloromethane,Tetrachloride, Carbon
D004195	Disease Models, Animal	Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases.	Animal Disease Model,Animal Disease Models,Disease Model, Animal
D004790	Enzyme Induction	An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.	Induction, Enzyme
D006427	Hemin	Chloro(7,12-diethenyl-3,8,13,17-tetramethyl-21H,23H-porphine-2,18-dipropanoato(4-)-N(21),N(22),N(23),N(24)) ferrate(2-) dihydrogen.	Ferriprotoporphyrin,Hematin,Alkaline Hematin D-575,Chlorohemin,Ferrihaem,Ferriheme Chloride,Ferriprotoporphyrin IX,Ferriprotoporphyrin IX Chloride,Panhematin,Protohemin,Protohemin IX,Alkaline Hematin D 575,Chloride, Ferriheme,Chloride, Ferriprotoporphyrin IX,Hematin D-575, Alkaline
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015427	Reperfusion Injury	Adverse functional, metabolic, or structural changes in tissues that result from the restoration of blood flow to the tissue (REPERFUSION) following ISCHEMIA.	Ischemia-Reperfusion Injury,Injury, Ischemia-Reperfusion,Injury, Reperfusion,Reperfusion Damage,Damage, Reperfusion,Injury, Ischemia Reperfusion,Ischemia Reperfusion Injury,Ischemia-Reperfusion Injuries,Reperfusion Damages,Reperfusion Injuries