A validated method for quantifying macrovesicular hepatic steatosis in chronic hepatitis C. 2007

Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
Department of Infectious Diseases, University Hospital of Wales, Cardiff, Wales, UK. tomboyles@yahoo.com

Hepatic steatosis is increasingly seen as an important prognostic factor in chronic hepatitis C infection (HCV). The commonly used semiquantitative method of measuring steatosis is based on a study that excluded patients with HCV. Several potentially useful methods of quantifying steatosis using computer-assisted morphometric analysis have been proposed, but none has been validated against a proposed gold standard other than the method they were intended to replace. We present a novel method and propose a gold standard based on manual measurements. The manual method is time consuming but shows little interobserver error, and the mean value of 3 observations by separate investigators is proposed as the gold standard. The computer-assisted method is fast, with a single interactive step that shows minimal interobserver variation. It accurately identifies biopsies with <1% steatosis (7 of 7) and predicts the gold standard value for biopsies with > 1% steatosis with narrow CIs (geometric mean ratio 0.85 with 95% CIs 0.77-0.95). This novel method of computer-assisted morphometric analysis is fast, reliable, and suitable for future research in HCV steatosis. It may be used to reanalyze previous studies. The semiquantitative method of assessing steatosis remains appropriate for clinical purposes.

UI MeSH Term Description Entries
D007091 Image Processing, Computer-Assisted A technique of inputting two-dimensional or three-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer. Biomedical Image Processing,Computer-Assisted Image Processing,Digital Image Processing,Image Analysis, Computer-Assisted,Image Reconstruction,Medical Image Processing,Analysis, Computer-Assisted Image,Computer-Assisted Image Analysis,Computer Assisted Image Analysis,Computer Assisted Image Processing,Computer-Assisted Image Analyses,Image Analyses, Computer-Assisted,Image Analysis, Computer Assisted,Image Processing, Biomedical,Image Processing, Computer Assisted,Image Processing, Digital,Image Processing, Medical,Image Processings, Medical,Image Reconstructions,Medical Image Processings,Processing, Biomedical Image,Processing, Digital Image,Processing, Medical Image,Processings, Digital Image,Processings, Medical Image,Reconstruction, Image,Reconstructions, Image
D005234 Fatty Liver Lipid infiltration of the hepatic parenchymal cells resulting in a yellow-colored liver. The abnormal lipid accumulation is usually in the form of TRIGLYCERIDES, either as a single large droplet or multiple small droplets. Fatty liver is caused by an imbalance in the metabolism of FATTY ACIDS. Liver Steatosis,Steatohepatitis,Steatosis of Liver,Visceral Steatosis,Liver Steatoses,Liver, Fatty,Steatohepatitides,Steatoses, Liver,Steatoses, Visceral,Steatosis, Liver,Steatosis, Visceral,Visceral Steatoses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D015588 Observer Variation The failure by the observer to measure or identify a phenomenon accurately, which results in an error. Sources for this may be due to the observer's missing an abnormality, or to faulty technique resulting in incorrect test measurement, or to misinterpretation of the data. Two varieties are inter-observer variation (the amount observers vary from one another when reporting on the same material) and intra-observer variation (the amount one observer varies between observations when reporting more than once on the same material). Bias, Observer,Interobserver Variation,Intraobserver Variation,Observer Bias,Inter-Observer Variability,Inter-Observer Variation,Interobserver Variability,Intra-Observer Variability,Intra-Observer Variation,Intraobserver Variability,Inter Observer Variability,Inter Observer Variation,Inter-Observer Variabilities,Inter-Observer Variations,Interobserver Variabilities,Interobserver Variations,Intra Observer Variability,Intra Observer Variation,Intra-Observer Variabilities,Intra-Observer Variations,Intraobserver Variabilities,Intraobserver Variations,Observer Variations,Variabilities, Inter-Observer,Variabilities, Interobserver,Variabilities, Intra-Observer,Variabilities, Intraobserver,Variability, Inter-Observer,Variability, Interobserver,Variability, Intra-Observer,Variability, Intraobserver,Variation, Inter-Observer,Variation, Interobserver,Variation, Intra-Observer,Variation, Intraobserver,Variation, Observer,Variations, Inter-Observer,Variations, Interobserver,Variations, Intra-Observer,Variations, Intraobserver,Variations, Observer
D019698 Hepatitis C, Chronic INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS. Chronic Hepatitis C
D022162 Cytoplasmic Vesicles Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism. Cytoplasmic Vesicle,Vesicle, Cytoplasmic,Vesicles, Cytoplasmic

Related Publications

Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
August 2015, La Revue de medecine interne,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
January 2008, Medical principles and practice : international journal of the Kuwait University, Health Science Centre,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
August 2002, Journal of gastroenterology and hepatology,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
November 2007, Japanese journal of infectious diseases,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
March 2004, JPMA. The Journal of the Pakistan Medical Association,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
May 2010, QJM : monthly journal of the Association of Physicians,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
January 2009, Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
January 2007, Hepatology (Baltimore, Md.),
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
January 2010, Journal of clinical gastroenterology,
Tom H Boyles, and Sarah Johnson, and Nigel Garrahan, and Andrew R Freedman, and Gerraint T Williams
July 2006, Gut,
Copied contents to your clipboard!