Surface myoelectric signal changes occurring during sustained isometric contractions have been extensively studied with quantitative surface electromyography (sEMG) and are described by means of some sEMG global variables in time and frequency domain (such as the median power spectral frequency). Recently, the possibility of studying local muscle O(2) saturation during exercise using non-invasive methods has been enhanced thanks to the use of near-infrared spectroscopy (NIRS). The purpose of this work was to combine NIRS and sEMG techniques to analyze the relationship between modifications of sEMG parameters and the underlying metabolic status of the exercising biceps brachii muscle. This relationship was tested under different isometric contraction modalities, namely static (ST) at 20, 40, 60 and 80%MVC and sinusoidal (SIN) at 40+/-20 and 60+/-20%MVC. Results clearly indicated the presence of an initial fast phase of muscle O(2) desaturation followed by a slow phase, regardless of the contraction modality. Moreover, the initial rate of muscle O(2) desaturation was related to the level of force output (R=0.92), but it was independent on the contraction modality (p<0.05). Similarly, changes in sEMG parameters were related to force level (Conduction Velocity-CV vs. Force: R=0.87; sEMG Median Frequency-MDF vs. Force: R=0.86). The high correlation found between CV-MDF and Tissue Oxygenation Index (TOI) slope (R=0.73 and 0.72, respectively) suggests a strong relationship between NIRS and sEMG data. This study indicates that muscle O(2) demand during isometric contractions from low to high force levels is influenced by the type of active motor units and not from the type of isometric exercise modality.