Fluorescence study on the interaction of bovine serum albumin with p-aminoazobenzene. 2008

Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
Department of Chemistry, College of Chemistry and Environmental Engineering, Yangtze University, JingZhou, Hubei 434025, People's Republic of China.

In this paper, the interaction between p-aminoazobenzene (PAAB) and BSA was investigated mainly by fluorescence quenching spectra, circular dichroism (CD) and three-dimensional fluorescence spectra under simulative physiological conditions. It was proved that the fluorescence quenching of BSA by PAAB was mainly a result of the formation of a PAAB-BSA complex. The modified Stern-Volmer quenching constant K(a) and the corresponding thermodynamic parameters DeltaH, DeltaG and DeltaS at different temperatures were calculated. The results indicated that van der Waals interactions and hydrogen bonds were the predominant intermolecular forces in stabilizing the complex. The distance r=4.33 nm between the donor (BSA) and acceptor (PAAB) was obtained according to Förster's non-radioactive energy transfer theory. The synchronous fluorescence, CD and three-dimensional fluorescence spectral results showed that the hydrophobicity of amino acid residues increased and the losing of alpha-helix content (from 63.57 to 51.83%) in the presence of PAAB. These revealed that the microenvironment and conformation of BSA were changed in the binding reaction.

UI MeSH Term Description Entries
D010128 p-Aminoazobenzene Used in the form of its salts as a dye and as an intermediate in manufacture of Acid Yellow, diazo dyes, and indulines. para-Aminoazobenzene,4-(Phenyldiazenyl)aniline,Aniline Yellow,C.I. Solvent Yellow 1,p Aminoazobenzene,para Aminoazobenzene
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002942 Circular Dichroism A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Circular Dichroism, Vibrational,Dichroism, Circular,Vibrational Circular Dichroism
D005453 Fluorescence The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012710 Serum Albumin, Bovine Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed) Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D013816 Thermodynamics A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed) Thermodynamic
D014364 Tryptophan An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals. Ardeydorm,Ardeytropin,L-Tryptophan,L-Tryptophan-ratiopharm,Levotryptophan,Lyphan,Naturruhe,Optimax,PMS-Tryptophan,Trofan,Tryptacin,Tryptan,Tryptophan Metabolism Alterations,ratio-Tryptophan,L Tryptophan,L Tryptophan ratiopharm,PMS Tryptophan,ratio Tryptophan

Related Publications

Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
January 2011, Luminescence : the journal of biological and chemical luminescence,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
February 2005, Guang pu xue yu guang pu fen xi = Guang pu,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
January 2004, Guang pu xue yu guang pu fen xi = Guang pu,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
January 2005, Journal of agricultural and food chemistry,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
July 2003, Analytical and bioanalytical chemistry,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
September 2017, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
March 2019, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
January 2010, Luminescence : the journal of biological and chemical luminescence,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
October 2010, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy,
Ye-Zhong Zhang, and Bo Zhou, and Yan-Xia Liu, and Chun-Xia Zhou, and Xin-Liang Ding, and Yi Liu
April 2009, Journal of fluorescence,
Copied contents to your clipboard!