Cloning the swamp buffalo SRY gene for embryo sexing with multiplex-nested PCR. 2007

Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
Animal Reproduction Institute, Guangxi Key Laboratory for Subtropical Bio-Resource Conservation and Utilization, Guangxi University, Nanning, Guangxi 530005, China.

The polymerase chain reaction (PCR) is an efficient method for sexing embryos. The objective of this study was to develop an accurate and reliable method for sexing swamp buffalo (Bubalus bubalis) embryos. The SRY gene from swamp buffalo genomic DNA was amplified by PCR, using primers based on the sequence of the Holstein SRY gene. This fragment was sequenced based on a BLAST search; the SRY gene was highly conserved. Using a Southern blot, there was a strong signal in genomic DNA only from male swamp buffalo. Two pairs of nested primers, targeted to amplify the swamp buffalo SRY conserved region, were designed for sex identification. Simultaneously, the G3PDH gene was co-amplified to serve as an internal control. A multiplex-nested PCR system was optimized by varying the following individually: concentrations of Mg(2+) and dNTPs, ratio of concentrations of primers and numbers of cycles. Biopsies of 27 IVF-derived embryos and 24 embryos fertilized with Y-chromosome-bearing sperm were examined. Using optimized procedures, clear signals following PCR amplification were obtained from all embryo samples; PCR amplification accuracy was further verified by comparing PCR and dot blots. We concluded that this PCR technique was highly reliable for sexing swamp buffalo embryos.

UI MeSH Term Description Entries
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D002020 Buffaloes Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus. Bubalus,Syncerus,Water Buffaloes,Buffalo,Water Buffalo,Buffalo, Water
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012732 Sex Determination Analysis Validation of the SEX of an individual by inspection of the GONADS and/or by genetic tests. Sex Determination Techniques,Sex Determination Technics,Analyses, Sex Determination,Analysis, Sex Determination,Sex Determination Analyses,Sex Determination Technic,Sex Determination Technique,Technic, Sex Determination,Technics, Sex Determination,Technique, Sex Determination,Techniques, Sex Determination

Related Publications

Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
March 1999, Theriogenology,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
October 1993, Molecular reproduction and development,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
March 1998, Molecular reproduction and development,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
December 2004, Animal genetics,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
July 2011, Theriogenology,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
February 1994, Science in China. Series B, Chemistry, life sciences & earth sciences,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
January 1993, Genetic analysis, techniques and applications,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
August 2004, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
October 2019, Parasitology research,
Q Fu, and M Zhang, and W S Qin, and Y Q Lu, and H Y Zheng, and B Meng, and S S Lu, and K H Lu
January 1988, Acta veterinaria Scandinavica. Supplementum,
Copied contents to your clipboard!