-transparent.png){kind=logo}
We have investigated the role of recombinant human interleukin-1 beta (rIL-1 beta) and recombinant human tumor necrosis factor alpha (rTNF-alpha) on PLA2 activity, protein synthesis and eicosanoid production in bovine pulmonary artery endothelial cells. Cellular PLA2 activity increased 4-fold and production of PGE2 increased 3-fold at 1-2 hrs in the presence of 10 units/ml rIL-1 beta. PLA2 activity increased 3-fold at 30 min and PGE2 production increased 2-fold with 5 x 10(-9) M rTNF-alpha. The data show that endothelial cells respond more rapidly to rIL-1 beta (2-6 hr) and rTNF-alpha (30 min) than do chondrocytes and synovial cells (6-16 hrs), suggesting endothelial cells may play a primary role in initiating the inflammatory response.
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
June 1989,
Agents and actions,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
January 1990,
Eicosanoids,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
June 1989,
Agents and actions,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
March 2023,
Biotechnology & genetic engineering reviews,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
September 1993,
Agents and actions,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
August 1996,
The Journal of biological chemistry,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
September 1994,
Infection and immunity,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
August 1990,
Inflammation,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
June 1993,
The Journal of biological chemistry,
R Goodman, and
T M Stevens, and
L R Mantegna, and
P R Kidd, and
R R Harris, and
J S Kerr
February 1988,
The Biochemical journal,
