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Adenylate kinase (EC 2.7.4.3) interferes positively in the serum creatine kinase (EC 2.7.3.2) assay when the rate of ATP production is monitored by a coupled enzyme system. A dual assay, measuring creatine kinase and adenylate kinase activity, was used to evaluate AMP and other possible adenylate kinase inhibitors that would permit specific measurement of creatine kinase activity in the presence of adenylate kinase. We found that AMP, routinely included in the creatine kinase assay system to inhibit adenylate kinase, partially inhibits both human serum creatine kinase and purified creatine kinase from rabbit muscle. The amount of creatine kinase inhibition is related directly to the AMP concentration and inversely to the substrate (ADP) concentration. We found that 25 mmol/liter of fluoride inhibits adenylate kinase without measurable effect on creatine kinase activity. We developed a serum creatine kinase assay including fluoride, and compared it with the dual assay system and with two commercial assay kits. Other halides or adenosine 2'-monophosphate did not selectively inhibit adenylate kinase.
T G Rosano, and
K J Clayson, and
P E Strandjord
March 1978,
Clinical chemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
February 1985,
Clinical chemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
October 2003,
Analytical biochemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
October 1987,
Biochemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
November 1976,
Clinical chemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
April 1973,
Proceedings of the National Academy of Sciences of the United States of America,
T G Rosano, and
K J Clayson, and
P E Strandjord
July 1978,
Clinical chemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
October 1965,
Analytical biochemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
October 1977,
Clinical chemistry,
T G Rosano, and
K J Clayson, and
P E Strandjord
June 1977,
The Journal of biological chemistry,
