[14C]Benzyl acetate is a potential radiotracer for the measurement of glial metabolism in the rat brain. 2007

Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
Division of Health Sciences, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan. momosaki@sahs.med.osaka-u.ac.jp

In order to develop a suitable radiotracer for the measurement of glial metabolism, we synthesized four different types of ester derivatives of [14C]acetate, namely, [14C]phenyl acetate, [14C]para-nitrophenyl acetate, [14C]2,4-dinitrophenyl acetate and [14C]benzyl acetate ([14C]BA), and evaluated their potencies in rats. Among the derivatives, the highest brain uptake at 30 s postinjection was observed for [14C]BA, which was more than 23 times higher than that of [14C]acetate itself. A long-term retention of [14C]BA radioactivity in the brain was observed, whereas rapid clearance of radioactivity was seen in the heart. [14C]BA was rapidly hydrolyzed in the intact rat brain, and less than 5% of radiolabeled parent was observed 1 min after the injection. Radiochemical analysis using thin-layer chromatography revealed that [14C]BA was rapidly converted to [14C]glutamine and [14C]glutamate in the cortex within 10 min after injection. Furthermore, the uptake of [14C]BA was significantly decreased following microinjection of fluorocitrate, a selective glial toxin. These results strongly suggest that [14C]BA may be a useful radiotracer for the measurement of glial metabolism in the intact rat brain.

UI MeSH Term Description Entries
D008297 Male Males
D008657 Metabolic Clearance Rate Volume of biological fluid completely cleared of drug metabolites as measured in unit time. Elimination occurs as a result of metabolic processes in the kidney, liver, saliva, sweat, intestine, heart, brain, or other site. Total Body Clearance Rate,Clearance Rate, Metabolic,Clearance Rates, Metabolic,Metabolic Clearance Rates,Rate, Metabolic Clearance,Rates, Metabolic Clearance
D009457 Neuroglia The non-neuronal cells of the nervous system. They not only provide physical support, but also respond to injury, regulate the ionic and chemical composition of the extracellular milieu, participate in the BLOOD-BRAIN BARRIER and BLOOD-RETINAL BARRIER, form the myelin insulation of nervous pathways, guide neuronal migration during development, and exchange metabolites with neurons. Neuroglia have high-affinity transmitter uptake systems, voltage-dependent and transmitter-gated ion channels, and can release transmitters, but their role in signaling (as in many other functions) is unclear. Bergmann Glia,Bergmann Glia Cells,Bergmann Glial Cells,Glia,Glia Cells,Satellite Glia,Satellite Glia Cells,Satellite Glial Cells,Glial Cells,Neuroglial Cells,Bergmann Glia Cell,Bergmann Glial Cell,Cell, Bergmann Glia,Cell, Bergmann Glial,Cell, Glia,Cell, Glial,Cell, Neuroglial,Cell, Satellite Glia,Cell, Satellite Glial,Glia Cell,Glia Cell, Bergmann,Glia Cell, Satellite,Glia, Bergmann,Glia, Satellite,Glial Cell,Glial Cell, Bergmann,Glial Cell, Satellite,Glias,Neuroglial Cell,Neuroglias,Satellite Glia Cell,Satellite Glial Cell,Satellite Glias
D011877 Radionuclide Imaging The production of an image obtained by cameras that detect the radioactive emissions of an injected radionuclide as it has distributed differentially throughout tissues in the body. The image obtained from a moving detector is called a scan, while the image obtained from a stationary camera device is called a scintiphotograph. Gamma Camera Imaging,Radioisotope Scanning,Scanning, Radioisotope,Scintigraphy,Scintiphotography,Imaging, Gamma Camera,Imaging, Radionuclide
D001921 Brain The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM. Encephalon
D002250 Carbon Radioisotopes Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes. Radioisotopes, Carbon
D005240 Feasibility Studies Studies to determine the advantages or disadvantages, practicability, or capability of accomplishing a projected plan, study, or project. Feasibility Study,Studies, Feasibility,Study, Feasibility
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001593 Benzyl Compounds Benzene derivatives that contain a benzyl group with the general formula Phenyl-CH2-R. Compounds, Benzyl
D014018 Tissue Distribution Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios. Distribution, Tissue,Distributions, Tissue,Tissue Distributions

Related Publications

Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
February 2016, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
January 1991, Chemical research in toxicology,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
July 1987, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
December 1986, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
February 2004, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
March 1967, Biochemistry,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
February 2009, Journal of magnetic resonance (San Diego, Calif. : 1997),
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
May 1981, Life sciences,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
January 1967, Journal of neurochemistry,
Sotaro Momosaki, and Rie Hosoi, and Tomoya Sanuki, and Kenichiro Todoroki, and Masatoshi Yamaguchi, and Antony Gee, and Osamu Inoue
January 2007, Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association,
Copied contents to your clipboard!