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A new form of high molecular weight DNA polymerase [EC 2.7.7.7] (polymerase N) was isolated from the nuclei of rat ascites hepatoma cells. Polymerase C, which was isolated previously from whole cell extract, was also isolated from the nuclei (Tsuruo, T. and Ukita, T. (1974) Biochim. Biophys. Acta 353, 146-159). Polymerase N was not found in the cytoplasmic fraction of the cell, while polymerase C existed in both the nucleus and cytoplasm. The molecular weight of polymerase N (8.7 S) was larger than that of polymerase C (7.4 S). On freezing and thawing, polymerase N was converted to polymerase C. In the nucleus the amount of polymerase N was larger than that of polymerase C. These data suggest that polymerase N, which was specifically present in the nucleus, was a complex form of polymerase C. In in vitro assay, polymerase N showed properties similar to those of polymerase C. Oligoribonucleotide was an effective initiator for the polymerization reaction by polymerase N. The DNA synthesis on single stranded fd phage DNA was greatly stimulated by the concomitant synthesis of RNA.
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
January 1976,
Journal of biochemistry,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
July 1973,
Biochemical and biophysical research communications,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
May 1989,
Biochimica et biophysica acta,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
January 1988,
Invasion & metastasis,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
January 1976,
Doklady Akademii nauk SSSR,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
November 1986,
Biochemistry international,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
October 1979,
Nucleic acids research,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
December 1975,
Acta medica Okayama,
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
January 1992,
Cellular and molecular biology (Noisy-le-Grand, France),
T Tsuruo, and
K Hirayama, and
H Satoh, and
T Ukita
October 1989,
Journal of biochemistry,
