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Studies with human bone marrow cells are often impaired by the poor quality of sternal aspirates due to varying numbers of contaminating blood cells before enrichment procedures and insufficient progenitor cell yields. In this study we report on experiments performed with human bone marrow cells isolated from a) spongiose bone fragments collected during hip surgery in patients with osteoarthritis or b) sternal aspirates. After Ficoll-Histopaque density gradient centrifugation absolute cell numbers were always lower in the samples obtained from sternal aspirates. However, both sources proved to yield the same proportions of the respective myeloid cell populations. Human long-term bone marrow cultures (LTBMC), semi-solid agar assays (CFU-GM day 14), and 3H-thymidine incorporation assays proved the comparability of the two bone marrow sources.
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
October 1991,
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P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
January 1982,
Journal of cellular physiology. Supplement,
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
August 1988,
Behring Institute Mitteilungen,
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
January 1990,
Haematologica,
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
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D Krumwieh
October 1991,
Experimental hematology,
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A Wilke, and
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E Rüde, and
D Krumwieh
January 1997,
Methods in molecular biology (Clifton, N.J.),
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
January 1990,
Methods in molecular biology (Clifton, N.J.),
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
March 1986,
The Journal of surgical research,
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
February 2001,
Bulletin of experimental biology and medicine,
P Kalla, and
P Hintz-Obertreis, and
A Wilke, and
P Griss, and
F R Seiler, and
E Rüde, and
D Krumwieh
August 1980,
Proceedings of the National Academy of Sciences of the United States of America,
