-transparent.png){kind=logo}
A hemolysin (designated Vm-rTDH) from Vibrio mimicus (AQ0915-E13) was purified by ammonium sulfate fractionation and successive column chromatography with DEAE-Sephadex A-25, hydroxyapatite, Mono Q, Superose 12 and Phenyl-Superose. The Mr of the subunit was estimated to be about 22,000 by sodium dodecyl sulfate-slab gel electrophoresis. The isoelectric point of Vm-rTDH was approximately pH 4.9. The hemolytic activity of Vm-rTDH was stable upon heating at 100 degrees C for 10 min, similar to that of the thermostable direct hemolysin (Vp-TDH) of V. parahaemolyticus. Vm-rTDH also showed lytic activities similar to those of Vp-TDH. Immunological cross-reactivity between Vp-TDH and Vm-rTDH was demonstrated by the Ouchterlony double-diffusion test. Thus we conclude that V. mimicus produces a newly discovered type of hemolysin (Vm-rTDH) which is similar to Vp-TDH.
H Yoshida, and
T Honda, and
T Miwatani
August 1986,
Canadian journal of microbiology,
H Yoshida, and
T Honda, and
T Miwatani
November 1973,
Infection and immunity,
H Yoshida, and
T Honda, and
T Miwatani
April 1986,
Infection and immunity,
H Yoshida, and
T Honda, and
T Miwatani
January 1988,
Methods in enzymology,
H Yoshida, and
T Honda, and
T Miwatani
January 1982,
Pharmacology & therapeutics,
H Yoshida, and
T Honda, and
T Miwatani
May 2010,
The Journal of biological chemistry,
H Yoshida, and
T Honda, and
T Miwatani
January 1986,
Canadian journal of microbiology,
H Yoshida, and
T Honda, and
T Miwatani
April 1988,
Infection and immunity,
H Yoshida, and
T Honda, and
T Miwatani
May 1997,
Infection and immunity,
H Yoshida, and
T Honda, and
T Miwatani
January 1976,
Recent advances in studies on cardiac structure and metabolism,
