Biomaterial Database

Determination of glycosylation sites using a protein sequencer and deglycosylation of native yeast invertase by endo-beta-N-acetylglucosaminidase. 1991

K Takegawa, and M Yoshikawa, and T Mishima, and M Nakoshi, and S Iwahara

Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.

Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae was tested for its capacity to release N-linked sugar chains from native yeast invertase. The enzyme liberated about 80% of the sugar chains from the native invertase. Deglycosylated invertase was digested by chymotrypsin or pepsin, and twelve N-acetylglucosamine-containing glycopeptides were isolated. The amino acid sequences of these glycopeptides were analyzed by a protein sequencer, and the elution position of 4-L-aspartylglycosylamine was directly identified by conventional sequencing. The endo-beta-N-acetylglucosaminidase was found to remove mainly nine sugar chains from native invertase.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002240	Carbohydrate Sequence	The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.	Carbohydrate Sequences,Sequence, Carbohydrate,Sequences, Carbohydrate
D006026	Glycoside Hydrolases	Any member of the class of enzymes that catalyze the cleavage of the glycosidic linkage of glycosides and the addition of water to the resulting molecules.	Endoglycosidase,Exoglycosidase,Glycohydrolase,Glycosidase,Glycosidases,Glycoside Hydrolase,Endoglycosidases,Exoglycosidases,Glycohydrolases,Hydrolase, Glycoside,Hydrolases, Glycoside
D006031	Glycosylation	The synthetic chemistry reaction or enzymatic reaction of adding carbohydrate or glycosyl groups. GLYCOSYLTRANSFERASES carry out the enzymatic glycosylation reactions. The spontaneous, non-enzymatic attachment of reducing sugars to free amino groups in proteins, lipids, or nucleic acids is called GLYCATION (see MAILLARD REACTION).	Protein Glycosylation,Glycosylation, Protein
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D012441	Saccharomyces cerevisiae	A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.	Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D017038	Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase	A group of related enzymes responsible for the endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose-content glycopeptides and GLYCOPROTEINS.	Di-N-Acetylchitobiosyl beta-N-Acetylglucosaminidase,Endo-beta-Acetylglucosaminidase,Endoglycosidase F,Endo D Endoglucosaminidase,Endo F Endoglucosaminidase,Endo H Endoglucosaminidase,Endo-N-Acetyl-beta-d-glucosaminidase,Endo-beta-N-Acetylglucosaminidase D,Endo-beta-N-Acetylglucosaminidase F,Endo-beta-N-Acetylglucosaminidase H,Endoglucosaminidase D,Endoglucosaminidase F,Endoglucosaminidase H,Endoglucosidase H,Endoglycosidase D,Endohexosaminidase F,Endohexosaminidase H,Peptide N-Glycosidase F,Di N Acetylchitobiosyl beta N Acetylglucosaminidase,Endo N Acetyl beta d glucosaminidase,Endo beta Acetylglucosaminidase,Endo beta N Acetylglucosaminidase D,Endo beta N Acetylglucosaminidase F,Endo beta N Acetylglucosaminidase H,Endo-beta-N-Acetylglucosaminidase, Mannosyl-Glycoprotein,Mannosyl Glycoprotein Endo beta N Acetylglucosaminidase,Peptide N Glycosidase F,beta-N-Acetylglucosaminidase, Di-N-Acetylchitobiosyl
D043324	beta-Fructofuranosidase	A glycoside hydrolase found primarily in PLANTS and YEASTS. It has specificity for beta-D-fructofuranosides such as SUCROSE.	beta-D-Fructofuranosidase,Acid Invertase,Invertase,Saccharase,Sacrosidase,Sucraid,Sucrose 6-Phosphate Hydrolase,beta-Fructosidase,6-Phosphate Hydrolase, Sucrose,Hydrolase, Sucrose 6-Phosphate,Invertase, Acid,Sucrose 6 Phosphate Hydrolase,beta D Fructofuranosidase,beta Fructofuranosidase,beta Fructosidase