-transparent.png){kind=logo}
A method to map sugars two-dimensionally for the analysis of the structures of oligosaccharides from glycosphingolipids is described. Nine neutral and ten acidic oligosaccharides were obtained from glycosphingolipids by endoglycoceramidase digestion and labelled with 2-aminopyridine. The pyridylamino oligosaccharides were clearly separated by high-performance liquid chromatography on commercially available C18-silica and amide-silica column. All compounds tested were mapped without any overlapping. The separation of the pyridylamino oligosaccharides on the C18-silica column depended on the numbers and positions of sialic acid and N-acetylhexosamine residues; on the amide-silica column, the separation depended on the total number of sugar residues.
K Ohara, and
M Sano, and
A Kondo, and
I Kato
May 1990,
Analytical biochemistry,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
October 2000,
Journal of chromatography. A,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
May 1994,
Analytical biochemistry,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
January 2000,
Methods in enzymology,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
January 1994,
Methods in enzymology,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
August 1991,
Carbohydrate research,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
January 1987,
Methods in enzymology,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
November 1973,
Journal of lipid research,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
April 2000,
Analytical chemistry,
K Ohara, and
M Sano, and
A Kondo, and
I Kato
October 1986,
Analytical biochemistry,
