Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems. 2008

Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
Department of Biochemical and Pharmaceutical Technology, University of São Paulo, Av. Prof. Lineu Prestes, 580 Bloco 16, Cidade Universitaria, 05508-000 São Paulo, Brazil. Hans-Olof.johansson@biochemistry.lu.se

The partition of hemoglobin, lysozyme and glucose-6-phosphate dehydrogenase (G6PDH) in a novel inexpensive aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The effect of NaCl and Na(2)SO(4), pH and PEG molecular size on the partitioning has been studied. At high pH (above 9), hemoglobin partitions strongly to the PEG-phase. Although some precipitation of hemoglobin occurs, high recovery values are obtained particularly for lysozyme and G6PDH. The partitioning forces are dominated by the hydrophobic and electrochemical (salt) effects, since the positively charged lysozyme and negatively charged G6PDH partitions to the non-charged PEG and the strongly negatively charged polyacrylate enriched phase, respectively.

UI MeSH Term Description Entries
D009113 Muramidase A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17. Lysozyme,Leftose,N-Acetylmuramide Glycanhydrolase,Glycanhydrolase, N-Acetylmuramide,N Acetylmuramide Glycanhydrolase
D011092 Polyethylene Glycols Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS. Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D005954 Glucosephosphate Dehydrogenase Glucose-6-Phosphate Dehydrogenase,Dehydrogenase, Glucose-6-Phosphate,Dehydrogenase, Glucosephosphate,Glucose 6 Phosphate Dehydrogenase
D006454 Hemoglobins The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements. Eryhem,Ferrous Hemoglobin,Hemoglobin,Hemoglobin, Ferrous
D000180 Acrylic Resins Polymers of high molecular weight which are derived from acrylic acid, methacrylic acid or other related compounds and are capable of being molded and then hardened to form useful components. Acrylic Resin,Resin, Acrylic,Resins, Acrylic

Related Publications

Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
October 1996, Journal of chromatography. B, Biomedical applications,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
January 1994, Biotechnology progress,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
January 1990, Biotechnology progress,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
January 2004, Biotechnology progress,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
April 2012, Journal of chromatography. A,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
October 2013, Bioprocess and biosystems engineering,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
September 1992, Journal of chromatography,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
June 1998, Journal of chromatography. B, Biomedical sciences and applications,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
November 1970, Biochimica et biophysica acta,
Hans-Olof Johansson, and Flavio Musa Magaldi, and Eloi Feitosa, and Adalberto Pessoa
January 2019, Preparative biochemistry & biotechnology,
Copied contents to your clipboard!