-transparent.png){kind=logo}
A neutral proteinase with endothelin (ET)-1 converting activity was identified in cytosol and membrane fractions prepared from rat lung, in a ratio of 1:4, respectively. The membrane-bound proteinase was solubilized by 0.5% 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) with an increase in specific activity, and then was characterized. The solubilized proteinase was capable of converting big ET-1 to ET-1 with an optimum pH of 6.5, and the conversion was dose-dependently suppressed by phosphoramidon (IC50 = 0.5 microM). The molecular mass of the proteinase was estimated to be about 500 kDa by gel filtration in the presence of 0.5% CHAPS. These results indicate that rat lung contains a phosphoramidon-sensitive neutral proteinase catalyzing conversion of big ET-1 to ET-1. The proteinase may be involved in the biosynthetic pathway of ET-1 in the lung and/or the conversion of circulating big ET-1.
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
February 1993,
European journal of pharmacology,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
February 1994,
The American journal of physiology,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
February 1993,
Biochimica et biophysica acta,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
January 1998,
Journal of cardiovascular pharmacology,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
January 1992,
Life sciences,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
April 1991,
Biochemical and biophysical research communications,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
March 2008,
Experimental lung research,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
January 1993,
Life sciences,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
November 1994,
Analytical biochemistry,
M Takaoka, and
K Shiragami, and
K Fujino, and
K Miki, and
Y Miyake, and
M Yasuda, and
G F Wang, and
K Hisaki, and
Y Matsumura, and
S Morimoto
January 1993,
Journal of cardiovascular pharmacology,
