BIOMAT Database Logo BIOMAT Database Logo

[Evaluation of the Sysmex NE-8000 analyzer according to the norms of the International Committee for Standardization in Hematology]. 1991

J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
Servei d'Hematologia Biológica, Hospital Clinic i Provincial, Universitat de Barcelona.

The Sysmex NE-8000 blood autoanalyser determines the values of red cells and platelets by impedance method and the differential leucocyte count (DLC) by means of radiofrequency plus two independent channels for eosinophils and basophils. The system is provided with a sampler capable of holding 100 closed tubes and its working velocity is about 120 samples per hour. The results of its evaluation are presented in this report. The parameters systematically provided by this cell counter include blood cell counts (plus haemoglobin rate, haematocrit and red cell indices), DLC platelet indices and volumetric distribution curves of red cells and platelets. The machine is provided with alarms on each of the above parameters for any suspicion of pathology. The accuracy analysis was performed with regard to the Technicon H*1 system and the conventional methods recommended by the ICSH. Precision, carry-over, linearity and effective velocity were evaluated in accordance to ICSH standards, and when assessing the DLC the number of visual revisions and the percentage of false positives and negatives were taken into account, and hence the sensitivity, specificity and efficiency of the machine. The accuracy of the parameters analysed was acceptable, except for MCHC and monocyte and eosinophil counts. A fair precision was found except for the monocyte count, along with excellent linearity except for high white-cell count. Less than 1.5% carry-over was observed, except for the leucocyte count. The number of necessary revisions of the DLC was somewhat higher than expected, the false negatives being below 11%. Sensitivity, specificity and efficiency were over 75%, except for hospital patients.(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D007390 International Agencies International organizations which provide health-related or other cooperative services. Agencies, International,Agency, International,International Agency
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D001772 Blood Cell Count The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES. Blood Cell Number,Blood Count, Complete,Blood Cell Counts,Blood Cell Numbers,Blood Counts, Complete,Complete Blood Count,Complete Blood Counts,Count, Blood Cell,Count, Complete Blood,Counts, Blood Cell,Counts, Complete Blood,Number, Blood Cell,Numbers, Blood Cell
D005188 False Negative Reactions Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed) False Negative Reaction,Reaction, False Negative,Reactions, False Negative
D005189 False Positive Reactions Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed) False Positive Reaction,Positive Reaction, False,Positive Reactions, False,Reaction, False Positive,Reactions, False Positive
D006405 Hematology A subspecialty of internal medicine concerned with morphology, physiology, and pathology of the blood and blood-forming tissues.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity

Related Publications

J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
An evaluation of the Sysmex NE-8000 hematology analyzer.
November 1991, American journal of clinical pathology,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
Evaluation of the Sysmex NE-8000. Automated hematology analyzer in a high-volume outpatient laboratory.
December 1993, American journal of clinical pathology,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
[Evaluation of the Sysmex NE-8000 hematologic autoanalyzer].
October 1991, Sangre,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
Evaluation of the automated haematology analyser Sysmex NE-8000.
May 1991, European journal of clinical chemistry and clinical biochemistry : journal of the Forum of European Clinical Chemistry Societies,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
[Comparative and simultaneous evaluation of three automated counters in hematology: Coulter STKS, Sysmex NE 8000, Technicon H-2].
January 1993, Annales de biologie clinique,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
[International Committee for Standardization in Hematology, ICSH].
April 1971, Casopis lekaru ceskych,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
[Hematology Standardization Committee. Recommendations of the International Council for Standardization in Hematology (ICSH) for the evaluation of hematologic autoanalyzers].
June 1996, Sangre,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
The characterisation of leukaemias with the Sysmex NE-8000.
January 1997, Acta haematologica,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
[On the International Committee for Standardization in Hematology: ICSH].
December 1968, Rinsho byori. The Japanese journal of clinical pathology,
J M Jou, and C Pastor, and M Aymerich, and N Villamor, and J C Reverter, and R M Brugués, and J L Vives Corrons
A data management software for the Sysmex NE 8000 haematology analyser.
July 1996, Computers in biology and medicine,
Copied contents to your clipboard!