BIOMAT Database Logo BIOMAT Database Logo

[Protein-free culture of esophageal cancer cell lines]. 1991

Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
First Department of Surgery, Faculty of Medicine, Kyoto University.

We have established 13 esophageal cancer cell lines capable of growing in a protein-free environment. The growth of these cells was not affected by conditioned medium, but the growth of NIH3T3 cells and human fibroblasts was stimulated by conditioned medium. On the other hand, conditioned medium inhibited the growth of human endothelial cells. Amplified int-2 oncogene correlated well with the growth of cells in a protein-free environment but the number of EGF receptors and growth effect of EGF did not relate to such growth. Esophageal cancer cells grow automatically, possibly involving mesenchymal cells via the paracrine system. This results in a poor prognosis in patients.

UI MeSH Term Description Entries
D009857 Oncogenes Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene. Transforming Genes,Oncogene,Transforming Gene,Gene, Transforming,Genes, Transforming
D011379 Prognosis A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations. Prognostic Factor,Prognostic Factors,Factor, Prognostic,Factors, Prognostic,Prognoses
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D004815 Epidermal Growth Factor A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form. EGF,Epidermal Growth Factor-Urogastrone,Urogastrone,Human Urinary Gastric Inhibitor,beta-Urogastrone,Growth Factor, Epidermal,Growth Factor-Urogastrone, Epidermal,beta Urogastrone
D004938 Esophageal Neoplasms Tumors or cancer of the ESOPHAGUS. Cancer of Esophagus,Esophageal Cancer,Cancer of the Esophagus,Esophagus Cancer,Esophagus Neoplasm,Neoplasms, Esophageal,Cancer, Esophageal,Cancer, Esophagus,Cancers, Esophageal,Cancers, Esophagus,Esophageal Cancers,Esophageal Neoplasm,Esophagus Cancers,Esophagus Neoplasms,Neoplasm, Esophageal,Neoplasm, Esophagus,Neoplasms, Esophagus
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D016895 Culture Media, Serum-Free CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells. Protein-Free Media,Serum-Free Media,Low-Serum Media,Culture Media, Serum Free,Low Serum Media,Media, Low-Serum,Media, Protein-Free,Media, Serum-Free,Media, Serum-Free Culture,Protein Free Media,Serum Free Media,Serum-Free Culture Media

Related Publications

Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Growth-regulatory mechanism of two human esophageal-cancer cell lines in protein-free conditions.
September 1993, International journal of cancer,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Serum-free culture of carcinoma cell lines.
January 1991, Cytotechnology,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Culture of Adherent Cancer Cell Lines.
January 2022, Methods in molecular biology (Clifton, N.J.),
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
[Establishment and characterization of human esophageal carcinoma cell lines--especially the role of the serum-free culture].
November 1991, Nihon Geka Gakkai zasshi,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Adaptation of cell lines to serum-free culture medium.
August 2003, Hybridoma and hybridomics,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Electrophoretic pattern changes in macrophage cell lines due to culture in protein-free medium.
June 1998, In vitro cellular & developmental biology. Animal,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Screening of single-cell clonal lines from Papilio demoleus Linnaeus cell lines for exogenous protein expression and adaptation in serum-free culture.
June 2020, In vitro cellular & developmental biology. Animal,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Protein-free fed-batch culture of non-GS NS0 cell lines for production of recombinant antibodies.
February 2007, Biotechnology and bioengineering,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
The usefulness of three-dimensional cell culture in induction of cancer stem cells from esophageal squamous cell carcinoma cell lines.
May 2013, Biochemical and biophysical research communications,
Y Shimada, and N Yamaguchi, and T Akiyama, and M Fukumoto, and T Wagata, and Y Kanda, and M Imamura, and T Tobe
Isolation and culture of prostate cancer cell lines.
January 2004, Methods in molecular medicine,
Copied contents to your clipboard!