-transparent.png){kind=logo}
A new sensitive and selective high-performance liquid chromatographic method for the analysis of desmosine and isodesmosine in human and rat tissues is described. This method requires a purification step with column chromatography, followed by precolumn derivatization phenylisothiocyanate. The reaction products are then separated by isocratic chromatography on a C18 column and quantitated by ultraviolet detection at 254 nm. The recovery of standards of both compounds added to tissue samples and analysed by this method is usually greater than 90%, and the absolute detection limit is 0.5 ng for both compounds. The method is sensitive enough to measure both substances in tissue fragments of 30 mg of wet mass, which means that it can be used to study elastin in small human biopsies.
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
February 1994,
Amino acids,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
January 1987,
Analytical biochemistry,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
March 1990,
Journal of chromatography,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
November 1987,
Journal of chromatography,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
October 1995,
Journal of chromatography. B, Biomedical applications,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
March 1989,
Journal of chromatography,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
April 1990,
Analytical biochemistry,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
April 1989,
Analytical biochemistry,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
March 2000,
Journal of chromatography. B, Biomedical sciences and applications,
M Salomoni, and
M Muda, and
E Zuccato, and
E Mussini
November 1982,
Journal of chromatography,
