An experimental infection with strain KOS/Haskovo of the Bovid Herpesvirus 2, isolated in Bulgaria, was induced in susceptible heifers through intradermal, intranasal, and intravenous application. Regardless of the route of infection clinically the disease manifested itself as an eczema of an urticarial type, accompanied by a febrile reaction, leukopenia, erythropenia, and oligochromemia. Highest titers of virus-neutralizing antibodies were established on the 30th day following infection in the i/v injected animals. All experimentally infected animals remained resistant to a challenge with a homologous virus on the 80th day after they were infected.