Introduction of new dentine bonding agents in a practice of dentistry, variety of results on their crucial characteristics in foreign literature and lack of information in our literature led to a necessity to conduct own scientific research on dentine adhesives in order to facilitate the choice of available products. The purpose of this study was to estimate the adhesive strength of different dentine bonding agents to a treated human dentine immediately after light curing of a compatible composite material. Shear and tensile bond strength of Tripton, Gluma, Scotchbond-2 and Dentin Protector was measured. Control material was glass-ionomer base-cement Vitrabond. Human extracted premolars from both jaws were prepared to a flat surface from either occlusal or buccal aspect, and materials were applied, respectively. The results showed: a) values of shear bond strength ranged from 2.01-3.92 MPa with no statistical differences between tested systems; b) tensile bond strength varied from 13.7-26-84 MPa; the highest value showed Scotchbond-2, following by Gluma, but significantly lower values showed Tripton and Dentin Protector; c) the aspect of prepared tooth crown had no significant effect on bond strength with the exception of shear bond strength for Gluma-system, when applied on buccal surface showed significantly higher values than applied occlusally; d) dentine-bonding agents which dissolve smear layer with acid components and remove it from prepared dentine surface showed stronger adhesion than agents which leave this debris on a dentin surface.