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A capillary gas chromatographic (GC) method for the simultaneous determination of urinary hippuric acid (HA) and o-, m- and p-methylhippuric acids (MHAs), metabolites of toluene and o-, m- and p-xylenes, respectively, is described. These metabolites are converted into their isopropyl derivatives by extractive alkylation with tetrahexylammonium ion as extracting agent and isopropyl bromide as alkylating reagent in benzene. The derivatives are analysed using a chromatograph equipped with hydrogen flame ionization detector, split injection system and DB-17 capillary column. Benzoylleucine is used as an internal standard. The derivatives are well separated within 5 min and no interfering peaks are observed. The calibration curves of HA and MHAs in the range 1-50 micrograms are linear and sufficiently reproducible for quantitative analysis. Urine can be analysed accurately and precisely by this method without prior clean-up of the sample.
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
August 1983,
Journal of chromatography,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
October 1986,
Journal of chromatography,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
January 1998,
La Medicina del lavoro,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
April 1973,
British journal of industrial medicine,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
November 1977,
British journal of industrial medicine,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
February 2001,
Journal of chromatography. B, Biomedical sciences and applications,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
January 1988,
IARC scientific publications,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
August 1991,
Bulletin of environmental contamination and toxicology,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
October 1969,
British journal of industrial medicine,
H Kataoka, and
K Manabe, and
S Nakase, and
M Makita
March 1986,
Sangyo igaku. Japanese journal of industrial health,
