Expression and localization of the mu-opioid receptor (MOR) in the equine cumulus-oocyte complex and its involvement in the seasonal regulation of oocyte meiotic competence. 2008

Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
Department of Animal Production, University of Bari, Valenzano-Bari, Italy. e.dellaquila@veterinaria.uniba.it

The micro-opioid receptor (MOR) was identified in equine oocytes, cumulus and granulosa cells. By RT-PCR, a 441bp fragment was observed. By immunoblotting, a 65 kDa band was detected in samples of winter anestrous whereas in cells recovered in breeding season, two bands, 65 and 50 kDa, were found. The 65 kDa band was significantly more intense in winter anestrous specimens. In samples recovered in the breeding season, this band significantly decreased with the raise of follicle size and was heavier in compact oocytes and cumulus cells. The protein was localized on the oolemma and within the cytoplasm of oocytes and cumulus cells. In vitro oocyte maturation rate (MR), analyzed by confocal microscopy for nuclear chromatin, microfilaments and microtubules, was reduced after the addition of 3 x 10(-8) M beta-endorphin in medium without additional hormones. Inhibitory effects of 10(-3) M Naloxone in oocytes collected in anestrous and spring transition were observed, both in presence and absence of hormones added to culture medium. Increased MRs were observed in oocytes collected in anestrous and cultured in presence of 10(-8) M Naloxone. The exposure to 10(-3) M Naloxone induced significant intracellular calcium increases in cumulus cells recovered all over the year. beta-Endorphin 3 x 10(-8) M induced significant calcium increases only in cumulus cells recovered in fall transition and anestrous. Naloxone 10(-8) M did not induce intracellular calcium modifications. We conclude that the MOR is differentially expressed in equine cumulus-oocyte complexes in the different seasons of the year and plays a role in the seasonal regulation of meiotic competence of equine oocytes.

UI MeSH Term Description Entries
D008540 Meiosis A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells. M Phase, Meiotic,Meiotic M Phase,M Phases, Meiotic,Meioses,Meiotic M Phases,Phase, Meiotic M,Phases, Meiotic M
D009270 Naloxone A specific opiate antagonist that has no agonist activity. It is a competitive antagonist at mu, delta, and kappa opioid receptors. MRZ 2593-Br,MRZ-2593,Nalone,Naloxon Curamed,Naloxon-Ratiopharm,Naloxone Abello,Naloxone Hydrobromide,Naloxone Hydrochloride,Naloxone Hydrochloride Dihydride,Naloxone Hydrochloride, (5 beta,9 alpha,13 alpha,14 alpha)-Isomer,Naloxone, (5 beta,9 alpha,13 alpha,14 alpha)-Isomer,Narcan,Narcanti,Abello, Naloxone,Curamed, Naloxon,Dihydride, Naloxone Hydrochloride,Hydrobromide, Naloxone,Hydrochloride Dihydride, Naloxone,Hydrochloride, Naloxone,MRZ 2593,MRZ 2593 Br,MRZ 2593Br,MRZ2593,Naloxon Ratiopharm
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D005260 Female Females
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D006736 Horses Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest. Equus caballus,Equus przewalskii,Horse, Domestic,Domestic Horse,Domestic Horses,Horse,Horses, Domestic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012621 Seasons Divisions of the year according to some regularly recurrent phenomena usually astronomical or climatic. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Seasonal Variation,Season,Seasonal Variations,Variation, Seasonal,Variations, Seasonal
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings

Related Publications

Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
January 2018, Reproduction, fertility, and development,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
September 2017, Molecular human reproduction,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
November 2014, Brain research,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
October 2013, Research in veterinary science,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
August 2010, Molecular reproduction and development,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
October 2022, Reproduction in domestic animals = Zuchthygiene,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
December 2003, The European journal of neuroscience,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
September 2009, Reproduction (Cambridge, England),
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
August 1997, Biology of reproduction,
Maria Elena Dell'Aquila, and Maria Albrizio, and Antonio Ciro Guaricci, and Teresa De Santis, and Filippo Maritato, and Jordi L Tremoleda, and Ben Colenbrander, and Lorenzo Guerra, and Valeria Casavola, and Paolo Minoia
January 2000, FEBS letters,
Copied contents to your clipboard!