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A bifunctional hapten was synthesized consisting of a blood group A active tetrasaccharide (A-tetra) and a blood group Le(a) active pentasaccharide lacto-N-fucopentaose II (LNF II), linked to each other with a phenylaminothiourea spacer connecting the reducing ends (A-tetra-LNF II). The hapten was demonstrated to retain both blood group A and Le(a) activity and could be easily bound to both monoclonal anti-A and anti-Le(a) affinity columns. Due to the strong temperature dependence of the two antibodies their binding to oligosaccharides, the bifunctional hapten could be utilized to achieve easy desorption in the final step of affinity purification of either monoclonal anti-Le(a) or anti-A. The system is postulated to have general applicability in affinity purification of any ligate that binds with an avidity too high to achieve non-denaturing desorption.
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
March 1984,
Journal of immunological methods,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
September 1990,
Journal of chromatography,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
January 1987,
Methods in enzymology,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
September 2014,
The Analyst,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
January 2000,
Methods in molecular biology (Clifton, N.J.),
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
January 1978,
Methods in enzymology,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
January 1995,
Biotechnology progress,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
January 1991,
Methods in enzymology,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
January 1990,
Allergie und Immunologie,
S Uppugunduri, and
J Dakour, and
E Kallin, and
T Norberg, and
D Zopf, and
A Lundblad
October 2009,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
