[Preservation of hearts by perfusion under pressure]. 1991

F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
Instituto do Coração do Hospital das Clínicas-FMUSP, São Paulo.

OBJECTIVE Development of method used to fix hearts in a reduced time, with preservation of their structures and characteristics. METHODS Fifty normal human hearts were fixed in a system with four reservoirs, a roller pump, PVC tubes and a plastic recipient. This system was used to maintain the continuous injection by pressure of formaldehyde solution, to the interior of the heart. RESULTS The fixation was satisfactory, pré-fixation time (13.93 +/- 5.13 min) and fixation time (60.53 +/- 12.50 min) were reduced related to previously utilized methods. CONCLUSIONS The method was good, with decrease of fixation time and obtention of fixed hearts, with preservation of their structures and characteristics.

UI MeSH Term Description Entries
D009926 Organ Preservation The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Organ Preservations,Preservation, Organ,Preservations, Organ
D010477 Perfusion Treatment process involving the injection of fluid into an organ or tissue. Perfusions
D011312 Pressure A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Pressures
D006321 Heart The hollow, muscular organ that maintains the circulation of the blood. Hearts
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000447 Aldehydes Organic compounds containing a carbonyl group in the form -CHO. Aldehyde
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D015925 Cryopreservation Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens. Cryofixation,Cryonic Suspension,Cryonic Suspensions,Suspension, Cryonic

Related Publications

F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
July 1989, Canadian journal of physiology and pharmacology,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
August 2014, Annals of transplantation,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
January 1991, The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
November 1968, Nature,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
October 1965, Archives of surgery (Chicago, Ill. : 1960),
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
May 1978, Transplantation,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
January 1988, Basic research in cardiology,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
September 1974, Transplantation proceedings,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
June 2007, The Journal of surgical research,
F B Jatene, and M H Magalhães, and P R De Oliveira, and A D Jatene
January 2001, Transplantation proceedings,
Copied contents to your clipboard!