Biomaterial Database

Whole-mount histochemical detection of beta-galactosidase activity. 2001

F A Pereira

Baylor College of Medicine, Houston, Texas, USA.

This unit describes fixation and staining for b-galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants. To help achieve consistent results with larger samples, an alternate protocol details preparation of thick sections. Support protocols are provided for clearing of tissues for better resolution and paraffin-embedding the whole embryo.

UI	MeSH Term	Description	Entries
D004622	Embryo, Mammalian	The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.	Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001616	beta-Galactosidase	A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.	Lactases,Dairyaid,Lactaid,Lactogest,Lactrase,beta-D-Galactosidase,beta-Galactosidase A1,beta-Galactosidase A2,beta-Galactosidase A3,beta-Galactosidases,lac Z Protein,Protein, lac Z,beta D Galactosidase,beta Galactosidase,beta Galactosidase A1,beta Galactosidase A2,beta Galactosidase A3,beta Galactosidases
D014021	Tissue Preservation	The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).	Preservation, Tissue,Preservations, Tissue,Tissue Preservations
D016591	Histocytological Preparation Techniques	Methods of preparing cells or tissues for examination and study of their origin, structure, function, or pathology. The methods include preservation, fixation, sectioning, staining, replica, or other technique to allow for viewing using a microscope.	Cytohistological Preparation Techniques,Cytohistologic Preparation Technics,Cytohistologic Preparation Technique,Cytohistologic Preparation Techniques,Cytohistological Preparation Technic,Cytohistological Preparation Technics,Cytohistological Preparation Technique,Histocytologic Preparation Technic,Histocytologic Preparation Technics,Histocytologic Preparation Technique,Histocytologic Preparation Techniques,Histocytological Preparation Technic,Histocytological Preparation Technics,Histocytological Preparation Technique,Preparation Technic, Cytohistologic,Preparation Technic, Cytohistological,Preparation Technic, Histocytologic,Preparation Technic, Histocytological,Preparation Technics, Cytohistologic,Preparation Technics, Cytohistological,Preparation Technics, Histocytologic,Preparation Technics, Histocytological,Preparation Technique, Cytohistologic,Preparation Technique, Cytohistological,Preparation Technique, Histocytologic,Preparation Technique, Histocytological,Preparation Techniques, Cytohistologic,Preparation Techniques, Cytohistological,Preparation Techniques, Histocytologic,Preparation Techniques, Histocytological,Technic, Cytohistologic Preparation,Technic, Cytohistological Preparation,Technic, Histocytologic Preparation,Technic, Histocytological Preparation,Technics, Cytohistologic Preparation,Technics, Cytohistological Preparation,Technics, Histocytologic Preparation,Technics, Histocytological Preparation,Technique, Cytohistologic Preparation,Technique, Cytohistological Preparation,Technique, Histocytologic Preparation,Technique, Histocytological Preparation,Techniques, Cytohistologic Preparation,Techniques, Cytohistological Preparation,Techniques, Histocytologic Preparation,Techniques, Histocytological Preparation,Cytohistologic Preparation Technic